BIOMEDICAL ASSAY LABORATORY FOR THE DIVISION OF INTRAMURAL POPULATION HEALTH RESEARCH

校内人群健康研究部生物医学检测实验室

• 批准号: 10011741
• 负责人: MICHAEL TSAI
• 金额: $ 1.76万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-10 至 2020-09-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10011741
• 关键词: Adipose tissue; Allergens; Asthma; Biocompatible Materials; Biological; Biological Assay; Biological Markers; Blood; Caring; Cell Array Analyses; Child Health; Collection; Concentration measurement; Contractor; Contracts; DNA; DNA purification; Data; Development; Diet; Enzyme-Linked Immunosorbent Assay; Extrinsic asthma; Follicular Fluid; Gene Chips; Genes; Hair; Health; Health behavior; Hormones; Human; Hypersensitivity; IgE; Immune Cell Activation; Intervention Studies; Laboratories; Leukotrienes; Measurement; Measures; Metabolic; Monitor; Nail plate; National Institute of Child Health and Human Development; Observational Study; Omentum; Phytoestrogens; Placenta; Plasma; Pregnant Women; Proteome; Proteomics; Protocols documentation; RNA; Reproduction; Research; Saliva; Scientist; Secure; Seminal fluid; Ships; Specimen; Spottings; Study Subject; T-Lymphocyte; Techniques; Temperature; Tissues; Umbilical Cord Blood; Urine; Whole Blood; adolescent health; cytokine; design; epidemiology study; flexibility; genome wide association study; metabolomics; population health; repository; reproductive; research study; sample collection; steroid hormone; urinary

The division of Intramural Population Health Research (DIPHR) conducts research on a variety of topics pertaining to human reproduction and development, child and adolescent health and behavior, and care of pregnant women, among other topics. Both observational and interventional research studies are designed and are implemented by DIPHR scientists, and most research includes collection of a diverse array of data and bio specimens. All bio specimens are collected using approved protocols and are de-identified prior to banking in NICHD-supported repositories. The contractor shall receive no identifying or personal health information about the study subjects. A wide range of specimen types are collected including whole blood, cord blood, buffy coats, plasma, serum, urine, saliva, tissues (e.g. placenta, omental fat), hair, nail clippings, follicular and seminal fluid, semen, and new born blood spots. DIPHR requires analysis of this diverse collection of specimen types for a multitude of factors, including concentrations of reproductive and other hormones; dietary biomarkers; cytokines; indicators of metabolic function including analysis; RNA isolation for use in expression arrays; and proteome analysis. DIPHR requires analysis of collected biological specimens including blood (plasma, serum, WBC, etc.), urine, saliva, adipose tissue, follicular fluid, seminal fluid, and other biologic material relevant for specific research initiatives. Proposed analyses include measurement of concentrations of steroid hormones; assay of total and allergen-specific IgE; expression array analysis of cell-sorted T cell subpopulations; extraction of DNA from whole blood and blood spots; measurement of urinary leukotrienes; purification of DNA from saliva; identification of naturally occurring phytoestrogens and metabolites; the flexibility to measure different analytes using multipex and ELISA techniques; and quantification of metabolomics, proteomics, and genome-wide association studies.

校内人口健康研究处（DIPHR）开展与人类生殖和发育、儿童和青少年健康和行为、孕妇护理等有关的各种主题的研究。观察性和干预性研究都是由DIPHR科学家设计和实施的，大多数研究包括收集各种数据和生物标本。