Acquisition of 500 MHz NMR Spectrometer

将购置一台500兆赫核磁共振波谱仪和计算机系统 用于结构生物学。 将开发新软件 用于分析NMR数据并利用该数据来确定 蛋白质在溶液中的结构 其他研究关注 线性或初级序列之间的关系 蛋白质和二级和三级fording蛋白质 三维空间 NMR数据将用于评估 了解潜在构象的方案 在不同的较大的氨基酸序列中含有相同的氨基酸序列， 氨基酸序列。 短DNA的结构和动力学 寡聚链和与DNA相互作用的小分子 蛋白质也将被研究。 导出的所述信息 核磁共振实验将与距离 几何计算、能量细化计算和 计算机图形学来生成溶液中的分子结构 比目前已知的更详细。 这些信息将被 与我们对蛋白质静态结构的了解相比， 从X射线衍射数据已知的DNA，并将导致更好的 理解建筑与 这些大分子的功能作用。

A 500 MHz NMR spectrometer and computer system will be acquired for use in structural biology. New software will be developed for analyzing NMR data and utilizing that data to determine the structure of proteins in solution. Additional studies concern the relationship between the linear or primary sequence of proteins and the secondary and tertiary fording of proteins in three dimensional space. NMR data will be used to evaluate schemes for understanding the potential conformations of identical sequences of amino acids contained in different larger amino acid sequences. The structure and dynamics of short DNA oligomeric chains and of small molecules that interact with DNA and protein will also be studied. The information derived from the NMR experiments will be used in conjunction with distance geometry calculations, energy refinement calculations and computer graphics to generate molecular structures in solution in greater detail than is presently known. The information will be compared to our knowledge of the static structure of proteins and DNA known from X-ray diffraction data, and will lead to a better understanding of the relationship between architecture and the functional role of these macromolecules.