Collaborative Research: Metabolic Manipulation of Taxus sp. Cell Cultures for Taxane Production

9625405舒勒首席调查员建议采用更合理的方法来优化从植物细胞培养中提取的新化合物的生产条件。他们计划通过使用代谢运输抑制剂、前体和信号化合物(如激发子和植物激素)的实验来利用植物中信号转导途径的不断发展的知识。这一方法将导致紫杉醇在红豆杉中合成、释放和降解的工作模型。细胞培养。PI认为，建议的方法应该可以推广到从植物细胞培养中提取的紫杉醇以外的化合物，并为生物化学工程师提供一种工具，以实现从植物细胞快速生产新的治疗化合物。***

9625405 Shuler The Principal Investigators (PIs) propose to fill the need for a more rational approach to optimization of production conditions for new compounds derived from plant cell cultures. They plan to exploit evolving knowledge of signal- transduction pathways in plants with experiments using metabolic transport inhibitors, precursors, and signaling compounds (e.g. elicitors and plant hormones). This approach is to lead to working models of TAXOL synthesis, release, and degradation in Taxus sp. cell cultures. The PIs believe the proposed approach should be generalizable to compounds other than TAXOL that are derived from plant cell cultures, and provide biochemical engineers a tool to achieve rapid production of novel therapeutic compounds from plant cells. ***