Acquisition of a Confocal Laser Scanning Microscope for Cell Biology and Biophysics

收购共聚焦激光扫描显微镜的细胞生物学和生物制药学项目摘要一笔赠款已授予博士彼得J.S.与位于伍兹霍尔的海洋生物实验室（MBL）的Smith合作，采购具有多光子激发能力的共聚焦激光扫描显微镜和图像分析系统。 该系统将安装在海洋实验室中央显微设施内，使广大的常驻研究人员，包括来自伍兹霍尔海洋学研究所的科学家、访问和暑期研究人员以及海洋实验室提供的许多研究生和博士后教育课程都可以使用。从基础生物学到生物医学科学。在MBL的居民社区中，这些包括：1。锥虫囊泡运输的研究。处理传染病的一个更大项目的一部分。Ca/CaM激酶II动力学在LTP中的作用一个更大的项目的一部分，调查记忆的基础; 3。单细胞真核极端微生物的生理学。研究适应性进化和蛋白质表达/修饰的更大项目的一部分。病原体的多药耐药性。5.有丝分裂纺锤体的结构动力学。一个关于细胞动态的实时成像的持续项目。神经元中的钙振荡。 几个正在进行的项目的一个组成部分，在细胞膜和胞质中寻找钙活性结构域。环境污染物对神经元发育的影响。MBL研究人员在毒理学领域正在研究的一个关键问题。红系细胞中铁转运的机制，这是MBL启动的新线粒体程序的一部分。MBL的科学家们对细胞动力学的研究一直很感兴趣，这必然需要对生命系统进行观察。 选择的显微镜最大限度地提高了这种能力。 选择系统组件以提供1）用于可见光谱中的不同荧光团的共焦荧光成像的能力; 2）多光子激发; 3）用于同时记录具有紧密间隔的发射光谱的多个荧光团的发射指纹识别; 4）足够范围的物镜，以使得能够对活细胞的生理学进行结构研究和实验; 5）精确定位荧光标记的结构; 6）研究中活细胞的温度控制; 7）充分利用共聚焦的数据采集能力的软件;以及8）用于图像分析和显示的软件。 补助金中提出的研究活动反映了MBL社区科学兴趣的多样性以及研究和教育的推广。 新的显微镜将是一个宝贵的除了MBL设施的尖端研究。 它对居民和游客的可用性（每年有200多名季节性调查人员访问MBL进行研究）将确保授予的赠款产生很大的影响和使用。

Acquisition of a Confocal Laser Scanning Microscope for Cell Biology and BiophysicsProject SummaryA grant has been awarded to Dr Peter J.S. Smith of the Marine Biological Laboratory (MBL), Woods Hole, for the acquisition of a confocal laser scanning microscope and image analysis system with multiphoton excitation capability. The system is to be housed in the MBL Central Microscopy Facility, making it available to a wide community of resident researchers, including scientists from the Woods Hole Oceanographic Institute, visiting and summer investigators, as well as by many of the graduate- and postdoctoral-level education courses offered at the MBLSeveral proposed research projects have already been formulated by the investigating team, spanning basic biology to the biomedical sciences. Amongst the resident community of the MBL these include:1. An examination of vesicle trafficking in trypanosomes. Part of a larger project dealing infectious diseases.2. The role of Ca/CaM kinase II dynamics in LTP. Part of a larger project investigating the foundations of memory; 3. The physiology of single-celled eukaryotic extremophiles. Part of a larger project to investigate adaptive evolution and protein expression/modification.4. Multidrug resistance in pathogens. 5. Architectural dynamics of the mitotic spindle. A continuing project on live imaging of cellular dynamics.6. Calcium oscillations in neurons. A component of several ongoing projects looking at calcium activity domains in the cell membrane and cytosol.7. The effects of environmental pollutants on neuronal development. A key issue being studied by MBL investigators in the field of toxicology.8. The mechanisms of iron trafficking in erythroid cells which forms part of a new mitochondrial program initiated at the MBL. Scientists at the MBL have had a long interest in the study of cellular dynamics, which by necessity requires observations on living systems. The microscope to be chosen maximizes this ability. System components were chosen to provide 1) capability for confocal fluorescent imaging of different fluorophores in the visible spectrum; 2) multiphoton excitation; 3) emission fingerprinting for simultaneous recording of multiple fluorophores with closely-spaced emission spectra; 4) a sufficient range of objective lenses to enable both structural studies and experiments on the physiology of living cells; 5) precisely localization fluorescent-labeled structures; 6) temperature control of live cells under study; 7) software to use fully the data acquisition capabilities of the confocal; and 8) software for image analysis and display. The research activities proposed in the grant reflect the diversity of scientific interest amongst the MBL community as well as outreach in research and education. The new microscope will be an invaluable addition the MBL facilities for cutting edge research. Its availability to residents and visitors alike (over 200 seasonal investigators visit the MBL for research purposes on an annual basis) will insure a high impact and use for the grant awarded.