Global repeat discovery of rye A and B chromosomes using a high-throughput 454 sequence survey

我们的目标是在全基因组范围内重建第一个具有代表性的黑麦高重复序列数据集。我们将使用高通量的454序列调查与其他草种的重复组成进行比较分析，以改进对大型基因组的分析。流动分选黑麦B染色体(B染色体)的序列分析将有助于深入了解B染色体的进化。将通过实验确定重复家系的染色体分布，以建立染色体特异性标记。这一分析将为高等真核生物产生快速基因组变化提供新的证据，特别是与自私染色体有关的变化。

We aim to reconstruct the first representative dataset of rye highly-repetitive sequences in silico on a whole-genome scale. We will use a high-throughput 454 sequence survey for comparative analysis of the repeat composition with other grass species, for an improved analysis of large genomes. The sequence analysis of flow-sorted rye B chromosomes (Bs) will allow insights in the evolution of Bs. The chromosomal distribution of repeat families will be determined experimentally to establish chromosome specific makers. This analysis will provide new evidence for the generation of rapid genome changes in higher eukaryotes, with particular relevance to selfish chromosomes.