Core Facility: Morphology - Imaging - Skeletal Characterization

我们的团队将作为表征任何来源的骨骼样本的核心设施。我们研究所在骨标本的表征方面拥有丰富的经验，包括成像方式(接触式x射线到μCT成像)、组织学(包括不脱钙处理)、组织形态计量学、骨质量评估(从QBEI的骨密度评估到μ-x射线元素分析)和功能测试(生物力学)。作为核心设施，我们将为基础和临床项目提供服务。对于基础研究项目，我们以最高的国际标准在细胞、结构和功能水平上为项目中使用的任何小鼠模型提供完整的骨骼特征。对于临床研究项目，我们提供完整的骨活检处理和评估，包括组织病理学诊断和组织形态计量学。此外，我们将不仅提供体外(XtremeCT)成像(μCT40)，还将以尽可能高的分辨率提供体内(μCT)成像。作为我们服务的一个组成部分，我们将进一步教授从事该项目任何项目的年轻科学家骨骼成像和骨骼形态。为了完成这项任务，我们将设立一年一度的暑期课程--骨形态-影像-生理学-病理学‘，向项目中的所有年轻科学家开放。此外，我们愿意在汉堡的实验室接待来访的科学家一段时间。这一核心设施将大大加强整个计划，因为所有参与的合作伙伴都将完全接触到汉堡的经验和骨骼形态、成像和病理学方面的基础设施。因此，SPP1468项目中所有骨骼样本的骨骼分析将达到最高的国际标准。此外，它将是具有成本效益的，因为这一核心设施使得为每个单独的项目建立骨成像和骨的不脱钙组织学处理所需的昂贵和复杂的基础设施变得可有可无。

Our group will serve as a core facility for characterization of bone samples of any origin. Our institute is equipped and has ample experince in the characterization of bone samples including imaging modalities (contact-x-ray to μCT-imaging), histology (including undecalcified processing), histomorphometry, bone quality assessment (from BMDD assement by qBEI to μ-xray element analysis) and functional testing (biomechanics). As a core facility we will provide service to both basic and clinical projects. For basic research projects we provide full skeletal characterization of any mouse model used in the program project on highest international standard at the cellular, structural, functional level. For clinical research projects we provide complete processing and evaluation of bone biopsies, including histopathological diagnosis and histomorphometry. Furthermore we will offer μCT imaging not only ex vivo (μCT40) but also in vivo (XtremeCT) at the highest possible resolution. As an integral part of our service we will further teach young scientist working in any project of the program in skeletal imaging and skeletal morphology. To accomplish this task we will establish an annual summer-course ‚Bone Morphology – Imaging – Physiology – Pathology’ open to all young scientist from the program project. Further we would offer to host visiting scientist in our labs in Hamburg for a period of time. This core facility will significantly strengthen the whole program as all partners involved will have full access to the Hamburg experience and infrastructure in bone morphology, imaging and pathology. Thus skeletal analysis of all bone samples within the program project SPP 1468 will meet highest international standards. Furthermore it will be cost effective as this core facility makes it dispensable to establish the expensive and complex infrastructure needed for both bone imaging and undecalcified histological processing of bone for each individual project.