I-Corps: High Frame-rate Fluorescence Lifetime Imaging Microscopy

高帧率荧光寿命成像显微镜系统承诺新的荧光成像能力，成本大大低于目前的系统。通过在单一平台上集成检测器和转换器，以低于1000美元的生产成本实现超过100帧/秒的帧速率应该是可能的。相比之下，每帧几秒钟的图像采集时间几乎是目前可用系统成本的100倍。该提案将帮助团队评估先前由国家科学基金会资助的研究的商业可行性。如果成功商业化，该团队将提供一项新技术，推动荧光寿命成像显微镜技术的发展。这一进步将允许荧光寿命的动态测量与廉价和简单的系统。这些测量结果可能会导致显微镜技术的进一步发展，以及新的医疗诊断系统的发展。除了广泛采用该系统可能带来的科学进步之外，将硬件从实验室过渡到生产也会产生积极的经济影响。

The High Frame Rate Fluorescence Lifetime Imaging Microscopy system promises new fluorescence imaging capabilities at costs dramatically lower than current systems. By integrating detectors and converters on a single platform, frame rates in excess of 100 frames/second at a production cost of under $1000 should be possible. This compares with image acquisition times of a few seconds per frame at near 100 times the cost fopr currently-available systems. This proposal will assist the team in assessing the commercial viability of the research that was previously sponsored by NSF. If successfully commercialized, the team will deliver a new technology that advances the state of the art in fluorescence lifetime imaging microscopy. This advancement will allow measurements of dynamics of fluorescence lifetimes with an inexpensive and simple system. These measurements could lead to further advances in microscopy as well as the development of new medical diagnostic systems. In addition to the scientific advances that would be possible with widespread adoption of the system, there would also be positive economic impacts that would result from transitioning the hardware from the lab and ramping production.