NSF/FDA Scholar in Residence Program on In Vitro Biological Characterization of 3D Printed Small-Scale Medical Devices

PI：Narayan，Roger J.Proposal：1445727标题：NSF/FDA学者驻留计划3D打印小型医疗设备的体外生物学特性意义本项目中获得的数据将作为材料特性-生物学性能数据库的基础，其中将考虑体外细胞-材料相互作用、蛋白质吸附和材料工艺参数之间的关系。调控科学方法将为复杂和创新的设备技术提供一系列方法和工具，以了解基本的组织-材料相互作用。技术说明在拟议的SIR计划中，一种称为多焦点双光子聚合的3D打印技术将被用于制造固体组织倒钩粘合剂，该粘合剂具有用于组织渗透的微尺度特征和用于氧化锆杂化材料细胞生长的亚微尺度特征。组织倒钩是一种创新的小型装置，在伤口闭合和愈合方面具有潜在的用途。他们将研究生物相容的光引发剂(例如，核黄素-三乙醇胺混合物和核黄素-精氨酸混合物)用于基于双光子聚合的3D打印。他们还将评估用于监测组织倒钩粘合剂中肌酐的电化学传感器的使用情况。在拟议的计划中，他们将与FDA的同事合作，使用适当的细胞(例如，真皮成纤维细胞和表皮角质形成细胞)来评估含有亚微米级毛孔的组织倒钩材料和肌酐传感器材料的体外生物兼容性。

PI: Narayan, Roger J.Proposal: 1445727Title: NSF/FDA Scholar in Residence Program on In Vitro Biological Characterization of 3D Printed Small-Scale Medical DevicesSignificanceThe data obtained in this project will serve as the basis for a material property-biological performance database, in which the relationship between in vitro cell-material interactions, protein adsorption, and material processing parameters will be considered. The regulatory science approach will provide a repertoire of methods and tools to understand the fundamental tissue-material interactions for complex and innovative device technologies.Technical DescriptionIn the proposed Scholar-in-Residence (SIR) program, a 3D printing technique known as multi-focus two photon polymerization will be used to create solid tissue barb adhesives with microscale features for tissue penetration and sub-microscale features for cell ingrowth out of a zirconium oxide hybrid material. Tissue barbs are innovative small-scale devices with potential use in wound closure and healing. They will examine use of biocompatible photoinitiators (e.g., riboflavin-triethanolamine mixtures and riboflavin-arginine mixtures) for two photon polymerization-based 3D printing. They will also evaluate incorporation of an electrochemical sensor for monitoring of creatinine within the tissue barb adhesive. In the proposed program, they will collaborate with FDA colleagues to evaluate the in vitro biocompatibility of tissue barb materials that contain sub-micrometer scale pores and creatinine sensor materials using appropriate cells (e.g., dermal fibroblasts and epidermal keratinocytes).