Two-dimensional high performance liquid chromatography in microfluidic chip devices

该项目的主要科学目标-微流控芯片设备中的二维高效液相色谱-是开发一种微型分析系统，用于通过基于多维芯片的色谱对复杂样品混合物进行快速分析表征。研究工作的中心方面是应用芯片实验室技术，实现两个压力驱动色谱柱与颗粒固定相的无死体积耦合。这种基于色谱柱高效耦合的方法将系统地研究多维高效液相色谱在微流控芯片中的巨大潜力。因此，应该有可能利用多维纳米高效液相的好处，并巧妙地绕过这项技术的技术障碍，这在文献中有很好的记录。在该项目中，开发了用于二维芯片层析的微流控系统，并将详细评估这一前景光明的技术的可能性和局限性。

The main scientific goal within the project -Two-dimensional high performance liquid chromatography in microfluidic chip devices- is the development of a miniaturized analysis system for fast analytical characterization of complex sample mixtures by multidimensional chip-based chromatography. Central aspect of the research work is the dead volume-free coupling of two pressure-driven chromatography units with particulate stationary phase by applying lab-on-a-chip technology. With this approach based on highly efficient coupling of chromatographic columns the great potential of multidimensional HPLC in microfluidic chips will be systematically investigated. Thereby, it should be possible to exploit the benefits of multidimensional nano-HPLC and to smartly bypass the technical hurdles of this technology, which are well documented in literature. Within the project microfluidic systems for two-dimensional chip chromatography are developed and the possibilities and limitations of this promising technology will be evaluated in detail.