Search for methods to control and treat allergy by regulating class switch recombination

以IgE产生的调控和类转换到IgA的调控为重点，通过干预类转换重组（class switch recombination， CSR）的位置或调控因子来操纵其靶基因特异性。在体外培养中，我们检测了特异性CSR是否可以通过Id2和Runx2/3等因子来控制，这些因子分别参与CSR对IgE和IgA的调节。我们还检测了这些因子是否能在细胞被诱导为IgE CSR后引导细胞向IgA转换。通过上述实验，验证了通过干预CSR的位置或调控因子来操纵CSR特异性的可能性，并可能成为通过消除IgE来控制过敏的靶点，并通过提高IgA的产生来加强对传染病的一线保护。

Focused on the regulation of IgE production and regulation of class switching to IgA, a trial to manipulate of the target gene specificity of class switch recombination (CSR) by intervening its place or regulatory factors was performed.It was examined whether the specific CSR could be controlled in an in vitro culture by the factors like Id2 and Runx2/3 which are known to be involved in the regulation of CSR to IgE and IgA, respectively. It was also examined whether such factors could guide the cells to switch to IgA after the cells were induced to IgE CSR.By the above experiments, a possibility was tested that intervening the place or regulatory factors of CSR to manipulate specificity of CSR and could be a target to control allergy by eliminating IgE and to strengthen first-line protection against infectious diseases by enhancing IgA production.