Morphological studies of the distribution of neuroactive peptides in the trigeminal and oral mucosa regions of the rat.

用免疫组织化学方法研究了口腔粘膜和牙髓两种组织的肽能神经支配，在口腔粘膜中，粘膜下层可见降钙素基因相关肽（CGRP）样免疫反应（IR）纤维和P物质（SP）样免疫反应（IR）纤维。免疫荧光双标法显示，这些纤维中大多数同时含有CGRP和SP，而牙髓中则含有CGRP-IR纤维。SP-IR纤维只是偶尔检测到。将固蓝（FB）注射到口腔粘膜和牙髓中。比较了两种情况下三叉神经节内FB标记的CGRP-IR神经元的细胞大小，颊粘膜内的FB标记CGRP-IR神经元多为小型，牙髓内的FB标记CGRP-IR神经元多为中型至大型。在出生时用辣椒素处理的大鼠中，口腔粘膜中含有CGRP和SP的纤维完全消失，但CGRP- ...更多信息 牙髓中IR纤维未受影响。结果表明，三叉神经节内SP、CGRP、亮氨酸脑啡肽（LE）、生长抑素（SOM）、胆囊收缩素-8（CCK-8）、神经降压素（NT）等神经元的分布均与正常口腔组织的分布相一致。用免疫组织化学方法观察了大鼠三叉神经脊束核（nVsp）内神经肽Y（NPY）和酪氨酸羟化酶（TH）的表达。在尾侧亚核的浅层，nVsp内神经肽的密度最高。这表明所研究的肽在热和伤害性信息的传导中起作用。另一方面，TH反应纤维在nVsp内分布较弥散，与神经肽反应纤维不同，nVsp尾侧亚核浅层的含肽细胞和纤维，尤其是SP和LE的分布模式相似，提示这些神经肽的共定位的可能性。nVsp内未见类免疫反应细胞，但免疫反应纤维密集分布于浅层。这些纤维被认为是初级传入。少

Peptidergic innervation of two types of oral tissues, oral mucosa and tooth pulp, was investigated by experimental immunohistochemistry.In oral mucosa, calcitonin gene-related peptide (CGRP)-like immunoreactive (IR) fibers and substance P (SP) IR fibers were observed in the submucosal layer. Some of these fibers were observed to enter the epithelium.The double labeling imunofluorescence method revealed that most of these fibers contain both CGRP and SP at the same time.On the other hand, CGRP-IR fibers were observed in the tooth pulp. SP-IR fibers were only occasionally detected. Fast blue (FB) was injected into the oral mucosa and into tooth pulp. Cell sizes of FB labeled CGRP-IR neurons in the trigeninal ganglia were measured and compared in these two cases.Most of the FB labeled CGRP-IR neurons were small in the buccal mucosa and medium to large in the tooth pulp. In rats treated at birth with capsaicin, CGRP and SP containing fibers in the oral mucosa totally disappeared, but CGRP- … More IR fibers were not affected in the tooth pulp. These findings suggest that these two types of oral tissues are innervated by different-sized CGRP-IR neurons in the trigeminal ganglia.The distribution of SP, CGRP, leucine enkephalin (LE), somatostatin (SOM), cholesystokinin-8 (CCK-8), neurotensin (NT). neuropeptide-Y (NPY) and tyrosin hydroxylase (TH) was investigated in the nucleus tractus spinalis nervi trigemini (nVsp) of the rat by means of immunohistochemistry. The highest density of the neuropeptides in the nVsp were observed in the superficial layer of the caudal subnucleus. This suggests that the peptides examined play roles in the conduction on modification of thermal and nociceptive information. On the other hand, TH reactive fibers were more diffusely distributed in the nVsp, which was quite different from those of neuropeptides.Distribution patterns of peptide containing cells and fibers, especially of SP and LE, in the superficial layer of the caudal subnucleus of nVsp were similar, suggesting the possibility of co-localization of these neuropeptides.CGRP-like immunoreactive cells were not observed in the nVsp but immunoreactive fibers were densely packed in the superficial layer. These fibers were considered to be primary afferents. Less