Structure analyses of dextran synthases

本研究旨在揭示低聚异麦芽糖合酶和水解酶的底物识别机制及其晶体结构。结合DGase（异氨寡糖水解酶）的晶体结构，提出了活性口袋底部的排水结构。我们研究了该水渠中定点诱变对该酶水解活性的影响。水解活性明显降低，但其结构没有变化。

We aimed to reveal the substrate recognition mechanism of isomaltooligosaccharide synthases and hydrolases with their crystal structures. With the crystal structure of DGase, isolamtooligosaccharide hydrolase, it is proposed that the water drain structure at the bottom of the active pocket. We study the effect of the site-directed mutagenesis in this water drain on the hydrolysis activity of this enzyme. The hydrolysis activity was decreased significantly without any difference in its structure.