Surveys on malaria and gene analysis in Southeast Asia

1999-2000年，在泰国、印度尼西亚、缅甸和越南进行了疟疾和G6 PD缺乏症的现场调查，采用吖啶橙子染色和快速诊断G6 PD缺乏症.在泰国，在Sangkhlaburiand拉农进行了疟疾和G6 PD调查。在拉农，检测到许多三日疟原虫感染和一些卵形疟原虫感染。在越南，在林东省宝禄进行了疟疾和G6 PD调查，疟疾患者人数似乎比以前增加。在印度尼西亚，我们调查了东努沙登加拉省弗洛雷斯岛Maumere和东爪哇省Kangean岛Pachitan的Banuwangi的疟疾和G6 PD。在Banuwangi，所有感染恶性疟原虫的患者都具有相同类型的SERA抗原基因，表明恶性疟原虫可能由一个患者传播.在缅甸，在孟邦、掸邦和若开邦的许多村庄进行了疟疾和G6 PD调查。在所有的村庄，我们发现许多感染三日疟原虫和一些与P. ovale。此外，在许多村庄发现了两种新的三日疟原虫。它们的形态与典型的三日疟原虫不同，具有不规则的早期滋养体和2-3个染色质颗粒。18 S核糖体RNA（SSUrRNA）基因序列也与已报道的典型三日疟原虫不同，表明这两种类型可能是三日疟原虫组中的新种。

During 1999-2000, field surveys of malaria and G6PD deficiency were conducted in Thailand, Indonesia, My anmar and Vietnam by using an acridine orange staining and a rapid diagnosis of G6PD deficiency.1. In Thailand, malaria and G6PD surveys were performed at Sangkhlaburiand Ranong. At Ranong, many infections with Plasmodium malariae and some with P.ovale were detected.2. In Vietnam, malaria and G6PD surveys were done at Bao Loc, Lam Dong Province, and numbers of malaria patients seemed to increase from before.3. In Indonesia, we surveyed malaria and G6PD at Maumere, Flores island, East Nusa Tenggara Province, and Banuwangi, Pachitan, Kangean island, East Jawa Province. At Banuwangi, all patients infected with P.falciparum possessed the same type of SERA antigen gene, indicating that falciparum malariamay be spread from one patient.4. In Myanmar, malaria and G6PD surveys were conducted in many villagesin Mon State, Shan State and Rakhine State. In all villages, we detected many infections with P.malariae and some with P.ovale. In addition, two new types of P.malariae were detected from many villages. Their morphologies were different from the typical P.malariae, having irregularshapes of early trophozoites and 2-3 chromatin granules. The 18S ribosomal RNA (SSUrRNA) gene was also different from the reported sequences of typical P.malariae, indicating that the two types may be new species in P.malariae group.