Establishment of Post-Proteomic Analysis by the Development of Video-Mass-Scope Method

1)灵敏质谱仪的研制：为了分析细胞分泌的分子，研制了离子阱-TOF质谱仪和带有纳米喷雾电离源的TOP质谱仪。2）分子捕获接口的研制：研究了纳米喷雾型离子捕集针尖，3）视频质谱仪的研制：通过设备的组合1.以及方法2，开发了视频质谱仪并将其应用于检测细胞分泌的分子。在肥大细胞刺激下，用该方法成功地检测了组胺和5-羟色胺。该研究在日本制药学会第123届年会上发表。4）单细胞MALDI-TOF质谱分析：该方法被开发并成功应用于根据细胞成熟过程分析单个肥大细胞中组胺含量的增加，并发表了报告。5）针纤维视频显微镜的开发：该范围也是为了长时间研究单个细胞而对细胞的辐射损伤很小而开发的。本研究为细胞行为及其分子含量的动态变化的直接、同步观测奠定了基础，并为细胞行为的实时监测奠定了基础。

1) Development of Sensitive Mass Spectrometer : In order to analyze the secreted molecules from a cell, an ion-trap-TOF mass spectrometer and a TOP mass spectrometer with Nano-spray ionization source were developed.2) Development of Molecular Trapping Interface : Nano-spray type ion trapping tips were investigated and the tip in which pre-concentrating resins were embedded inside, was developed and applied to pre-concentration of secreted molecules from Mast cells.3) Development of the Video-Mass-Scope : By combination of the equipments 1. and method 2., the video-mass-scope was developed and applied to the detection of secreted molecules from the cells. On the stimulation of Mast Cells, Histamine and Serotonin were successfully detected by this method. This research was presented in the 123-th Annual Meetings of Japan Pharmaceutical Society.4) Single Cell MALDI-TOF Mass Spectrometric Analysis : This method was developed and successfully applied to analyze the increase of Histamine contents in a single Mast Cell according to the cell maturation process and the report was published.5) Development of Pin Fiber Video-Micro-Scope : This scope was also developed in order to investigate a single cell for long time with little irradiation damage to the cell. This method is also found to be useful to apply to the single molecular detection.The fundamentals and related methodologies were successfully developed by this study and the potentials of these methods were high enough to the direct and simultaneous observation of cell behavior and its dynamic changes in molecular contents.