A structure scientific study about interaction of polyamine and left-handed DNA.

我了解到N1-(2-(2-aminoethylamino)ethyl)ethane-1，2-diamine PA(2222)和N1-(2-(2-(2-aminoethylamino)ethylamino)ethyl)ethane-1，2-diamine PA(2222)非常容易连接在Z-DNA的一个小沟槽中，并且非常稳定地作为一个小沟槽结合剂。此外，我在4摄氏度、10摄氏度、20摄氏度的高盐密度下与这11种多胺和d(CG)3形成络合物，给出了20摄氏度的盐密度并结晶。我研究了多胺的Z-DNA的作用，通过研究这些来详细地研究稳定作用。我对Z-DNA的一条长链进行结晶，如果我了解稳定机制，有人说上述结晶相对于八个碱基到目前为止是困难的。我认为要弄清楚B-Z转换m…的DNA结构，应该是B型dna和Z型dna的混合型d(CGCGCGCGAATTCGCGCGCG)2、d(CGCGCGAATTCGCGCG)2和d(CGCGAATTCGCGCG)2更多的机制，Z-B转换机制。关于B-Z混合型DNA的结构，国际上到目前为止都在尝试结晶，但除了我们的结晶实例外，还没有报道。我想让我们掩埋一个大的小凹槽的空腔，它是在一个不稳定的元素中，这个多胺和多胺是一个小凹槽的结合剂，作为DNA和这些长链的复合体，整个DNA的稳定结构。因此，我们成功地对长链的左手DNA进行了X射线结晶学分析。关于B-Z混合型DNA的结构，国际上到目前为止都在尝试结晶，但除了我们的结晶实例外，还没有报道。我发现它爬行的凹槽结合剂多胺连接在Z-DNA的一个小凹槽中，我埋了一个很大的小凹槽，稳定了整个DNA的结构。应用这一点，我进行了长链DNA的结晶，B-Z-B结结构的结晶。我认为，如果我现在已经用这种方法成功地结晶了世界上最长的DNA，并且较少按顺序阐明长链DNA的结构或B-Z-B结结构，那么我在DNA结构研究方面将会有很大的发展

I understood that N1-(2-(2-aminoethylamino)ethyl)ethane-1,2-diamine PA (222) and N1-(2-(2-(2-aminoethylamino)ethylamino)ethyl)ethane-1,2-diamine PA (2222) were easy to be connected in a minor groove of Z-DNA very much and stabilized Z-DNA as a minor groove binder very much. Furthermore, I crystallize at 4 degrees Celsius, 10 degrees Celsius, 20 degrees Celsius by the high salt density with these 11 kinds of polyamine and d(CG)3 as a complex and give the salt density at 20 degrees Celsius very much and crystallize. I examine a role for Z-DNA of polyamine, stabilization action in detail by examining these. I perform crystallization of a long chain of Z-DNA which it has been said to that the above-mentioned crystallization vs. eight bases is difficult till now if I understand stabilization mechanism. I think to clarify structure of DNA such as mixture type d(CGCGCGCGCGAATTCGCGCGCGCG)2, d(CGCGCGCGAATTCGCGCGCG)2 and d(CGCGCGAATTCGCGCG)2 of B type DNA and Z type DNA to clarify B-Z transfer m … More echanism, Z-B transfer mechanism. As for the structure of DNA of B-Z mixture type, crystallization is tried all over the world till now, but there is not a report besides an example of our crystallization. I think about letting we bury a cavity of the big minor groove that it is it in an element of destabilization with this polyamine with the polyamine which is a minor groove binder as DNA and a complex of these long chains and stabilize structure of the whole DNA. Therefore we succeed in X-ray crystallographic analysis of left-handed DNA of a long chain. As for the structure of DNA of B-Z mixture type, crystallization is tried all over the world till now, but there is not a report besides an example of our crystallization. The groove binder polyamine which it is crawled, and I discovered is connected in a minor groove of just Z-DNA and I bury a big minor groove and stabilize structure of the whole DNA. I apply this and perform crystallization of DNA of a long chain, crystallization of B-Z-B junction structure. I think that I bring big development in structured study of DNA if I have already succeeded in crystallization of the DNA which is the longest in the world with this method now, and structure or B-Z-B junction structure of DNA of a long chain are clarified in sequence Less