Active multi-spectral aberration free phase microscope

目前的研究是基于过去关于“科学研究资助”的研究结果。本研究的目的是利用主动动态空心锥照明和数字（数字）8形带通滤波器构建无像差焦深度扩展相显微镜。同时，多光谱成像能力的确认也是本研究的重要课题。为此，在本研究的第一阶段（1997）对光学显微镜进行了改造，并安装了主动动态空心锥照明器。编写了照明器控制、图像处理和网络实用程序，并对该系统进行了测试，成功地确定了该系统作为无像差焦深扩展相显微镜的功能。通过对多个样品的分析，除多光谱成像外，该系统的实用性较好。下一阶段（1998年），建立并报道了基于有源动态空心锥照明和8字形带通滤波器的成像技术。本理论表明，主像差和高阶球像差都得到了成功的校正，并扩展了焦深。理论还表明，由于这种成像技术只观察到整个图像的平移，主彗差的影响也可以忽略不计。另一方面，对于多光谱成像，实验并没有很好地实现。2种颜色染色样品在白光光源下的多光谱观察显示分色不足。这种分色不足，可能是由于非线性成像效应和图8形带通滤波器在不同波长下的固有交叉。前者降低了高、中位空间频率范围内的频谱分辨率，后者降低了低空间频率范围内的频谱分辨率。目前，对这种分色不足的分析和对策正在进行中。最后，该成像技术在透射电镜上的应用是本研究的一部分。然而，由于机器时间的限制，利用透射电子显微镜的实验现在被安排在未来。少

The present study is based on results of past studies of 'Grant-in-aid for scientific research'. The aim of this study is the construction of aberration-free focal depth extended phase microscope using active dynamic hollow-cone illumination and figure (numerical) 8 shaped band-pass filter. And, the confirmation of the ability of the multi-spectral imaging is also important subject of this study.According to this aim, an optical microscope was reconstructed to attach an active dynamic hollow-cone illuminator at the first stage (1997) of this study. Programs for the illuminator control, the image processing and the network utility were also coded in this time, This system was then examined and successfblly confirmed its functions as the aberration-free focal depth extended phase microscope. Using several samples, the practicability of this system is evaluated as to be good except for the multi-spectral imaging.In the next stage (1998), the basic theory was established and reported for t … More he present imaging technique based on the active dynamic hollow-cone illumination and the figure 8 shaped band-pass filter. The present theory shows that principal and higher order spherical aberrations are, all, successfully corrected in addition to the extension of the focal depth. And the theory also shows that the influence of the principal coma aberration can be also neglected since only the entire image translation is observed using this new imaging technique.On the other hand, the experiment is not well achieved, as to the multi-spectral imaging. The multi-spectral observation of 2 color dyed sample under white light source shows insufficient color separation. This insufficient color separation is, probably, due to the non-linear imaging effect and the intrinsic crossing of the figure 8 shaped band-pass filter for different wave length. The former degrades the spectral resolution at high and median spatial frequency range, and the later degrades at the lower spatial frequency range. The analysis and the countermeasure of such insufficient color separation are, now, in progress.Finally, the application of this imaging technique to the transmission electron microscope is the part of present study. The experiment using transmission electron microscope is, however, now scheduled in future due to the restriction of the machine time. Less