New Methods for Directed Molecular Evolution of Novel Protein Functions

基于核糖体的新技术可以用于进行具有新结构和功能的蛋白质的试管进化。这些方法依赖于(A)单个核酸分子与它们编码的特定蛋白质分子的物理联系，(B)选择具有新功能的蛋白质，以及(C)恢复附着的遗传密码。该项目将解决目前限制这些前沿技术用于进化具有广泛实际应用的新蛋白质产品的几个问题。

Novel ribosome-based techniques can be used to carry out test-tube evolution of proteins with new structures and functions. The methods rely on (a) physical association of individual nucleic acid molecules with the particular protein molecules they encode, (b) selection of proteins with new functions, and (c) recovery of the attached genetic code. This project will address several issues that currently limit use of these frontier technologies for evolution of new protein products that have a wide range of practical applications.