Study on active synthetic imaging method for microscope

本研究的目的是建立新提出的主动合成成像方法的理论背景和应用，在作者以前发展的主动图像处理的基础上，在第一阶段（1990年），主动合成成像方法的基础理论研究已经进行了使用的三维光学传递函数（3D OTF）的概念。通过在传统光学显微镜上附加光学/机械部件，构建了一个原型光学系统。该原型系统的3D功率谱已被测量使用一个新开发的3D图像采集/处理系统。这些结果表明，有效的三维OTF的“合成”是真正可能的。另一方面，还通过使用3D图像采集/处理系统测量了透射电子显微镜图像的3D功率谱。这些光谱是在透射电子显微镜领域的首次观测。 ...更多信息 y.实验结果表明，主动合成成象法在透射电镜中是很好的应用方法，下一阶段（1991年）提出了无球差观察法并进行了实验。该方法是基于移动离焦平均的主动合成成像方法的一种应用。此外，还研制了一套真实的实时（2-4 TV帧频）主动合成成像系统，并成功地应用于无相位板相位显微术。另一方面，离焦调制型非实时球差校正也已应用于日立HF-2000透射电子显微镜。实验结果表明，该方法的分辨率提高了1.6-1.7倍，可望在光学显微镜和透射电子显微镜领域得到广泛的应用。少

The present study has aimed to establish the theoretical background and the application of the newly proposed active synthetic imaging method, under the basis of the active image processing previously developed by authors.At the first stage(1990), basic theoretical studies on the active synthetic imaging method have been carried out using the concept of the 3D optical transfer function(3D OTF). And a prototype optical system has been constructed by attaching additional optical/mechanical parts to a conventional optical microscope. 3D power spectra of this prototype system have been measured using a newly developed 3D image acquisition/processing system. These results show that the 'synthesis' of the effective 3D OTF is truly possible. On the other hand, the 3D power spectra of the transmission electron microscope images have also been measured by using the 3D image acquisition/processing system. These spectra are the first observation in the field of the transmission electron microscop … More y. From these measurements, it has been also concluded that the active synthetic imaging method is well applicable in the transmission electron microscopy.Proposal and experiment of the spherical aberration free observation method have been done at the next stage(1991). This method is based on the moving defocus averaging as an application of the active synthetic imaging method. In addition to this, a prototype of the real time(2-4 TV frames rate)active synthetic imaging system has been constructed and successfully applied to the phase plate-less phase microscopy. On the other hand, the non-real time spherical aberration correction of defocus modulation type has also applied to a Hitachi HF-2000 transmission electron microscope. The experimental results show 1.6-1.7 times improvement of the resolution.From present studies, it is expected that the present active synthetic imaging method can be widely applied to both fields of the optical microscopy and the transmission electron microscopy. Less