Development of serological diagnostic kit for summer-type hypersensitivity pneumonitis.

由丝孢酵母菌属引起的夏季型过敏性肺炎（S-HP）是日本最常见的过敏性肺炎类型，最近在韩国也发现了这种情况。为了方便和简化S-HP的诊断，我们试图建立一种能够评价血清中特异性抗体活性的ELISA方法。我们得到了以下结果。1.从小鼠腹水中纯化出特异性抗病原抗原的单克隆抗体，该单克隆抗体可作为捕获抗体.用单克隆抗体偶联亲和层析柱纯化丝孢酵母种特异性抗原。利用该亲和柱，可以获得足够量的抗原，用于建立ELISA检测系统.比较了丝孢酵母属特异性抗原葡糖醛酸甘露聚糖固定于酶标板上的酶联免疫吸附试验（ELISA）和酶联免疫吸附试验（ELISA）。经实际样品测量，前者灵敏度高，节省时间.

Summer-type hypersensitivity pneumonitis (S-HP) caused by Trichosporon is the most prevalent type of hypersensitivity pneumonitis in Japan, and recently it is also discovered in Korea. In order to facilitate and simplify the diagnosis of S-HP,we attempt to establish sandwich-ELISA system which enables evaluation of specific antibody activity in serum. We obtained the following results.1. Purification of monoclonal antibody (Mab) specific for causative antigenisity was done from ascites of mice, and this Mab can work well as capture antibody.2. Purification of Trichosporon species specific antigen by monoclonal antibody coupled affinity column was done. By using this affinity column, we can obtain an enough amount of antigen to make sandwich-ELISA system.3. We compared with sandwich-ELISA system and ELISA system in which Trichosporon species specific antigen, glucuronoxylomannan, was chemically fixed to ELISA plate. After measured samples, the former system yielded higher sensitivity and time saving.