Experimental model for studying health promotion and preventive medicine using rats.

生活方式是影响健康状况的主要因素。为预防肥胖、高血压、糖尿病等疾病，应向医务人员建议适当的生活方式，包括饮食、环境温度、休息方式。本研究的目的是建立一个实验模型，为研究健康生活方式对预防疾病和促进健康的作用提供实验依据。Wistar大鼠用戊巴比妥钠（50 mg/kg ip）麻醉，将动脉和静脉导管分别插入右股动脉和静脉。然后，还将左肾腹膜后暴露，并在手术显微镜的帮助下，将通向肾的交感神经的分支解剖出来，并放置在精细的不锈钢双极电极上。将热电偶（铜-康铜）放置在腹腔内用于测量核心体温。同时植入用于脑电图和肌电测量的电极。恢复期后至少2天开始实验，研究饮食、环境温度、睡眠时间等生活方式的改变对心血管、自主神经和能量代谢功能的影响。

Life style is a major factor influencing health status.causing. To prevent diseases such asobesity, hypertension, and diabetes, proper way of living including meal, environmental temperature, resting manner should be suggested by medical professional. However, little information is available on this issue at present.The purpose of the present study is to establish a experimental model for studying the proper life style to prevent disease and to promote the health. Wistar rats was anesthetized with pentobarbital sodium (50 mg/kg ip), and arterial and venous catheters were inserted into the right femoral artery and vein, respectively. Then, the left kidney was also exposed retro-peritoneally and with the aid of an operation microscope, a branch of the sympathetic nerves to the kidney was dissected free and placed on a fine stainless steal bipolar electrode A thermocouple (copper-constantan) was placed in the intra-abdominal space for the measurement of core body temperature. The electrodes for the measurements of electroencephalogram and electromusculargram were also implanted. At least 2 days after the recovery period, experiment started.This methods allows us to study the effect of the changes in life style including meal, environmental temperature, sleep time, on the cardiovascular, autonomic and energy metabolism functions.