Molecular biological study of pathogenesis of the Summer-Type Hypersensitivity Pneumonitis

在此之前，我们曾报道过毛孢子菌属是夏季过敏性肺炎(SHP)的主要病原体，SHP是日本最流行的过敏性肺炎(HP)类型。在这项研究中，我们重新评估了丝孢子菌属内的三种抗原型作为毛滴虫的病原体。相应的标准株TIMM 1573(血清型I)、TIMM 1318(血清型II)和M9456(血清型III)分别被归类为粘液毛滴虫、阿萨氏毛霉和运动毛霉，根据每种培养方式和其他遗传、生理和形态特征的DNA/DNA关联度达到或超过95%。为了确认这些血清型的重要性，对从患者环境中分离的98株其他毛孢子菌和24株CBS菌株进行了血清分型，其中包括该属内描述的物种的模式培养。220例重型肝炎患者血清抗毛孢子虫抗体分析。的抗原性。目前的结果是…S进一步指出，毛孢子虫病最常见的病原是阿萨氏毛滴虫和粘毛毛滴虫，它们似乎是引起SHP的主要病原体。我们还应用聚合酶链式反应和Southern杂交技术检测了小鼠模型或SHP患者的支气管肺泡灌洗液中以及患者家中空气中的抗原特异性DNA。吸入后6h和96h，模型组BALF阳性率分别为100%和60%。18例患者入院后1周内BALF阳性率为94%。23例其他疾病患者和7例健康志愿者的总有效率分别为22%和29%。空气样品由Andersen型小流量空气采样器采集。采样板上的颗粒物用超声波清洗器采集并经PCR放大，2/2的空气样本呈阳性。利用聚合酶链式反应本身或附加的限制性内切酶Sau3 AI，可将阿萨氏毛滴虫和粘液毛滴虫的信号与其他真菌或人类细胞区分开来。用聚合酶链式反应检测宿主和环境中的病原体特异性DNA，将是研究微生物暴露所致职业病发病机制的一种可扩展的方法。较少

Previously, we reported that the genus Trichosporon was a major causative agent of summer-type hypersensitivity pneumonitis(SHP), the most prevalent type of hypersensitivity pneumonitis (HP) in Japan. In this study, we re-valued three antigenic types within the genus Trichosporon as the causative agents of SHP.Corresponding standard strains, TIMM 1573 (serotype I), TIMM 1318 (serotype II), and M9456 (serotype III) were assigned to species T.mucoides, T.asahii and T.motevideense, respectively, based on 95% or more DNA/DNA relatedness with each type of culture and other genetical, physiological and morphological characteristics. To confirm the significance of these serotypes, 98 other strains of Trichosporon isolated from patients' environments and 24 CBS strains, including type cultures of species described within the genus, were serotyped. Serum antibody analysis of 220 SHP patients against Trichosporon spp. were also examined to estimate the antigenic profile of SHP.The present result … More s indicate that T.asahii and T.mucoides, which are the most common causes of trichosporonosis, appear to be the major causative agents of SHP.We also applied polymerase chain reaction (PCR) and Southern hybridization to detect the antigen specific DNAs in the bronchoalveolar lavage fluid (BALF) of a murine model or patients with SHP, and in the air of patients' homes. BALF samples of the model were positive in 100 and 60% at 6 and 96 h after the inhalation challenge, respectively. The BALF samples of 18 patients obtained within 1 week after admission were positive in 94%. Those of 23 patients with other diseases and seven Healthy, volunteers were 22 and 29%, respectively. Air samples were collected by an Andersen type low volume air sampler. Particles on the sampling plates were harvested by a ultrasonic cleaner and amplified by PCR.The 2/2 air samples were positive. The signal of each of T.asahii and T.mucoides were discriminated from the other fungi or human cells by PCR itself or the additional restriction enzyme, Sau3 AI.The detection of pathogen specific DNAs in the host and in the environment by PCR would be an expandable method for the investigation of the pathogenesis of occupational diseases caused by microbial exposures. Less