A flow cytometry suite for multiscale biosorting

该提案旨在建立一个最先进的流式细胞仪套件，这将使澳大利亚首次在一个地点分离最大范围的生物样本，从病毒到植物种子和动物胚胎。流式细胞仪套件将包括三台最先进的流式细胞仪(1)一台2激光Union Biometrica BioSorter大颗粒流式细胞仪，用于大型物体（如胚胎）；（ii） 5激光BD Biosciences FACSAria融合细胞分选仪与FSC PMT用于中小型物体（如细胞），（iii） 2激光NanoFCM流动纳米分析仪用于最小的生物物体（如病毒，囊泡）。这些新的广泛能力将支持农业、兽医和微生物科学的研究。

The proposal aims to establish a state-of-the-art flow cytometry suite that will allow for the first time in Australia at one site, the separation of the broadest range of sizes of biological samples from viruses, to plant seeds and animal embryos. The flow cytometry suite will include three cutting edge flow cytometers (i) a 2 laser Union Biometrica BioSorter large particle flow cytometer for large objects (eg embryos); (ii) a 5 laser BD Biosciences FACSAria Fusion cell sorter with FSC PMT for small to mid-size objects (eg cells), (iii) a 2 laser NanoFCM flow nanoanalyzer for the smallest biological objects (eg viruses, vesicles). These new broad capabilities will support research in agricultural, veterinary and microbiological sciences.