The role of histidine on wound healing using cultured rat intestinal epithelial cells

在病理条件下，生物体内的氨基酸平衡会因各种应激而失去平衡。本研究的目的是利用培养的大鼠肠上皮细胞(RIE)研究病理条件下的重要氨基酸组氨酸在创伤愈合中的作用。在缺乏组氨酸的培养基和零培养基中，RIE的恢复速度明显延缓。在四甲基偶氮唑盐比色法中，与全培养液相比，细胞在缺乏组氨酸的培养液中孵育24小时后细胞增殖显著降低。HSP70和裂解的Caspase-3在缺乏组氨酸的培养基和零培养基中的表达均高于全培养基组。提示热休克蛋白的诱导、细胞凋亡的诱导和生长因子的减少参与了组氨酸缺乏导致创面延迟愈合的机制。

The amino acid balance in the living body will lose a balance for various stresses under pathological condition. The aim of this study was to investigate the functional role of histidine which is the important amino acid in pathological conditions on wound healing using cultured rat intestinal epithelial cells (RIE). In the histidine lack culture medium and the Zero culture medium, the restitution speed of RIE showed delay remarkably. In MTT assay, compared with the Full culture medium, the cell proliferation after 24 hour incubation in the histidine lack culture medium was significantly decreased. In the histidine lack culture medium and the Zero culture medium, the expression of HSP70 and Cleaved Caspase-3 were increased, compared with the Full culture medium. It was suggested that the induction of heat shock protein, the induction of apoptosis and the reduction of growth factor were involved in the mechanisms of delayed wound healing by lack of histidine.