Detection of Nobel Food Components for Preventing Altheimer's Disease and Evidence of its Action Mechanizum

微管系统的损伤对于解释阿尔茨海默病(AD)的发病机制具有重要意义。我们报道了磷脂酰胆碱(PO)对PC12细胞微管形成的抑制作用，这可能是神经变性疾病早期脑细胞的一个很好的模型。这一事实表明PO可能是AD的原因之一。微管组装系统依赖于暴露在氧化应激下的细胞中的抗氧化酶系统。提示抗氧化剂可能是预防AD的有效药物。在我们的实验中，PC12细胞是在PO变质后使用的。添加抗坏血酸、表儿茶素或β-胡萝卜素使活细胞数增加，并使抗氧化酶活性恢复。

The impairment of the microtubule systemis important to explain the pathogenesis of Alzheimaer's disease(AD). We have reported on the inhibition of microtubule formation by phosphatidylcholine hydroperoxides(PO) in PC12 cells, which may be a good model of brain cells in the early stages of neurodegenerative diseases. This fact indicates that PO may be one of the cause for AD. The microtubule assembly system was dependent on the antioxidative enzyme system in cells exposed to oxidative stress. It is suggested that antioxidant agents may be effective in preventing AD. In our experiments, PC12 cells were used after deterioration by PO. The additon of ascorbic acid, epicatechin or beta-carotene made increase of alive cell number, and made to recover the activities of antioxidative enzymes.