NMR study for the whole structure of human general transcription factor TFIIE

一般转录因子是RNA聚合酶II启动蛋白质编码基因转录所必需的。在本研究中，我利用NMR对人类TFIIE这一通用转录因子成员的整体结构进行了结构分析。人类TFIIE是一种异源二聚体，由α和β亚基组成。TFIIE分子量大（~80kDa），有聚集倾向。尽管这些特征不利于核磁共振结构分析，但利用最新的核磁共振技术和高磁场核磁共振仪可以获得TFIIE三个功能域的结构信息。我能够在完整的TFIIE复合体中确定三个功能域之一的高分辨率三级结构。

General transcription factors are necessary for transcription initiation of protein coding genes by RNA polymerase II. In this study, I structurally analyzed the whole structure of human TFIIE, a member of the general transcription factors by using NMR. Human TFIIE is a heterodimer, consisting of an alpha and a beta subunit. TFIIE has large molecular weight (~80kDa) and shows the tendency to aggregate. Although these characters are disadvantage for structural analysis by NMR, use of the latest NMR technologies and high magnetic filed NMR machines enabled to gain structural information on three functional domains of TFIIE. I was able to determine a high resolution tertiary structure of one of three functional domains in the intact TFIIE complex.