Study of cell-cell interaction: Cell manipulation method for tissue regeneration

我们开发了一种通过将膜系单链寡核苷酸与光学透明玻璃底物结合的互补单链寡核苷酸杂交的方法，用于细胞的二维图图化，这使我们能够表征长期培养的图图化细胞。在含有fbs的培养基中浸泡的图案底物显示允许粘附血清蛋白的吸附，这允许在最初仅通过DNA杂交附着的细胞中传播和参与自然细胞骨架粘附模式。我们发现，当细胞试图从最初的附着点迁移时，共存的附着模式会导致膜结合系索和自然细胞骨架粘附机制之间的竞争。这种竞争最终以细胞从其指定的模式中逃逸而告终，并在脂质插入物对细胞膜的亲和力上“赢得”细胞骨架迁移力。

We developed a method for the 2-dimensional patterning of cells via hybridization of membrane-tethered single stranded oligonucleotides to complimentary single stranded oligonucleotides bound to optically transparent glass substrates which allowed us to characterize the long term culture of patterned cells. Patterned substrates immersed in FBS-containing media are shown topermit the adsorption of adhesive serum proteins which allowed for the spreading and engagement of natural cytoskeletal adhesion modes in cells initially attached only through DNA hybridization. We show that the coexisting modes of attachment result in competition between membrane-bound tethers and natural cytoskeletal adhesion machinery as cells attempt to migrate away from their initial points of attachment. This competition ends in the escape of cells from their designated patterns and the ‘winning out’ of cytoskeletal migration forces over the affinity of lipid inserts for the cell membrane.