Purchase of Affymetrix GeneTitan for use in high throughput genotyping

请拨资金用于购买基因泰坦多通道基因分型系统和5年保修。购买基因泰坦的申请是由布里斯托基因组学设施的学术主任基思爱德华兹教授发起的，此前他与布里斯托及其他地区的几位研究人员和工业合作伙伴进行了讨论。将直接受益于在布里斯托放置GeneTitan的研究人员包括Eric Morgan博士在布里斯托尔对动物健康和牲畜生产的研究，阿曼达丘吉尔博士在布里斯托尔对老龄化和幸福的研究，Ian King教授在伯明翰对粮食安全的研究，Andy Greenland教授在NIAB对粮食安全的研究，教授博士朱莉威廉姆斯'卡迪夫为基础的研究与衰老和博士彼得杰克的小麦育种在RAGT的疾病过程中，以解决与粮食安全相关的问题。所有这些案例都是除了基思爱德华兹教授自己对小麦育种及其与粮食安全关系的广泛研究之外。支持这些科学案例的所有科学案例都需要高通量/高密度基因分型; GeneTitan能够在全基因组基因分型应用所需的规模上实现这一点。GeneTitan通过将所需组件（杂交炉、射流处理和最先进的成像设备）组合到单个台式仪器中，实现从靶杂交到数据生成的自动阵列处理。因此，GeneTitan通过最大限度地减少用户干预，并允许无人值守，夜间并行处理大量样品，为每个操作员提供最高的吞吐量和实验室生产力。这种程度的免手动自动化意味着数据是一致可重复的，特别是基因表达研究的一个基本属性，并意味着手动阵列处理通常花费的时间可用于数据分析，增加了获得有意义研究结果的时间。

Funds are requested to purchase both a GeneTitan-Multi Channel (GeneTitan) genotyping system and a 5-year warranty. The application to purchase a GeneTitan has been instigated by Professor Keith Edwards, the academic director of the Bristol Genomics Facility, following discussions with several researchers and industrial partners both at Bristol and beyond. Researchers who would directly benefit from the placing of a GeneTitan at Bristol include Dr Eric Morgan's Bristol-based research into animal health and livestock production, Dr Amanda Churchill's Bristol-based research into ageing and wellbeing, Professor Ian King's Nottingham-based research into food security, Professor Andy Greenland's research into food security at NIAB, Professor Dr Julie Williams' Cardiff-based research into the disease processes associated with ageing and Dr Peter Jack's wheat breeding at RAGT to address issues associated with food security. All of these cases are in addition to Professor Keith Edwards' own extensive research into wheat breeding and its relationship to food security.The scientific cases for support all require high throughput/high density genotyping; the GeneTitan enables this on a scale required for genome wide genotyping applications. The GeneTitan achieves this by automating array processing from target hybridization to data generation by combining the required components (hybridization oven, fluidics processing, and state-of-the art imaging device) into a single bench-top instrument. As such the GeneTitan provides the highest throughput and laboratory productivity per operator by minimizing user intervention and allowing unattended, overnight processing of large numbers of samples in parallel. This degree of hands-free automation means that data is consistently reproducible, an essential attribute of gene expression studies in particular, and means that the time typically taken for hands-on array processing is available for data analysis increasing the time to meaningful results for research.