SALTED FISH AND NASOPHARYNGEAL CARCINOMA

这项建议是为了进行一系列合作研究，涉及 北京大学流行病学学部和化学致癌学部 南加州综合癌症中心和卫生部 麻省理工学院营养与食品科学专业。 这项研究的总体目标是阐明病因。 进食广式咸鱼与健康的关系 华南地区鼻咽癌的发病情况 这是我们最近在香港进行的病例对照研究的结果。 我们将在广州进行类似的病例对照研究，人民的 中国共和国在香港确认调查结果。案件将会是 年50岁以下鼻咽癌组织学确诊病例 诊断。控制将是年龄(在5年内)和性别匹配的 邻里控制。我们计划完成300对病例对照 采访。我们将进行一个对照动物实验，其中148个 近交系Wistar-京都大鼠将被喂食咸鱼粉和大鼠 确定广式咸鱼是否为 在这个动物模型中是致癌的。我们会进行实验室研究，以 测定咸鱼提取物引起突变的能力 哇巴因对8-氮鸟嘌呤耐药及诱发肿瘤的作用 哺乳动物细胞的转化。我们会进行实验室研究，以 分离和鉴定盐渍鱼提取物中形成的化合物 模拟胃条件下亚硝化后的细菌诱变剂 条件。已被鉴定为具有致突变性的化合物 因子分析将在哺乳动物细胞分析中重新测试，包括 培养二倍体人包皮成纤维细胞的实验研究 对人类细胞造成遗传毒性。

This proposal is for a series of collaborative studies involving the Epidemiology Unit and the Chemical Carcinogenesis Unit of the Unviersity of Southern California Comprehensive Cancer Center and the Department of Nutrition and Food Science at the Massachusetts Institute of Technology. The overall objective of this research is to elucidate the etiologic relationship between ingestion of Cantonese-style salted fish and the development of nasopharyngeal carcinoma (NPC) in southern Chinese as suggested by the findings of our recent case-control study in Hong Kong. We will conduct a similar case-control study in Guangzhou, People's Republic of China to confirm the findings in Hong Kong. Cases will be histologically confirmed incident cases of NPC under age 50 at the time of diagnosis. Controls will be age- (within 5 years) and sex-matched neighborhood controls. We plan to complete 300 case-control pair interviews. We will conduct a controlled animal experiment in which 148 inbred Wistar-Kyoto rats will be fed a diet of powdered salted fish and rat chow mixture to determine whether Cantonese-style salted fish is carcinogenic in this animal model. We will conduct laboratory studies to determine the ability of salted fish extracts in causing mutation to ouabain and to 8-azaguanine resistance and in inducing neoplastic transformation in mammalian cells. We will conduct laboratory studies to isolate and identify the compounds in salted fish extracts which form bacterial mutagens following nitrosation under simulated gastric conditions. Compounds that have been identified as mutagenic in the facterial assays will be retested in mammalian cell assays including cultured diploid human foreskin fibroblasts to characterixe their ability to cause genotoxicity in human cells.