Proteins in motion - new tools for biotechnology

该项目旨在通过使用新的核磁共振（NMR）波谱技术监测蛋白质的运动来评估蛋白质的功能。由于晶体学已经确定了三维蛋白质结构的快照，新工具被设计用于分析溶液中功能重要的运动。一个超快（> 100 kHz）半固态蛋白质魔角旋转核磁共振波谱设备将为澳大利亚带来尖端技术，并允许对选定蛋白质区域的3D结构和动力学进行调查。该项目的预期结果将对生物催化剂的合理工程和药物开发中先导化合物的设计产生直接的好处。

This project aims to assess the function of proteins by monitoring their motions using new nuclear magnetic resonance (NMR) spectroscopy techniques. As snapshots of 3D protein structures have been determined by crystallography, the new tools are designed to analyse functionally important motions in solution. A facility for ultrafast (> 100 kHz) magic angle spinning NMR spectroscopy of proteins in the semi-solid state will bring cutting-edge know-how to Australia and allow the interrogation of 3D structure and dynamics in selected protein regions. The expected outcomes of the project will have immediate benefits for the rational engineering of biocatalysts and in the design of lead compounds in drug development.