Dissecting a RNA-histone variant interaction and its role in splicing

该项目旨在确定染色质成分组蛋白H2A.B如何与RNA结合并影响RNA剪接的分子细节。这对于组蛋白来说是史无前例的，组蛋白通常与DNA和转录调控有关。超过90%的人类基因可能是选择性剪接的。这解释了复杂的生物体是如何从有限的一组基因发展而来的，但如何做出替代剪接决定尚不清楚。其预期结果是揭示染色质、RNA剪接和基因表达调控之间的联系，以解释多细胞生物是如何进化的。这些知识的转化最终将为澳大利亚带来长期的经济和健康利益。

This project aims to define the molecular details of how a chromatin component, histone H2A.B, binds RNA and influences RNA splicing. This is unprecedented for histones, which are typically associated with DNA and transcriptional regulation. Over 90 per cent of human genes may be alternatively spliced. This explains how complex organisms develop from a limited set of genes, but how alternative splicing decisions are made is unclear. The intended outcome is to reveal links between chromatin, RNA splicing and gene expression regulation to explain how multicellular organisms have evolved. The translation of this knowledge will ultimately provide long-term economic and health benefits for Australia.