ELECTROCHEMICAL SENSORS FOR ENZYME IMMUNOASSAYS

该项目的总体目标是开发高度稳定的 用于检测还原辅因子的电化学探针 烟酰胺腺嘌呤二核苷酸(NADH)，它在许多 基于酶的免疫分析。这些传感器可以测量NADH浓度。 在低外加电位下，电化学干扰和 避免了使化学反应失活。第一阶段的研究将 试图确定使用这些新传感器的可行性 临床重要免疫分析中产生的NADH的测定。这个 一种可选择性测量的电化学探头的研制 NADH浓度可以分析非常小的样品，这 例如，对新生儿血液的临床分析具有重要意义 样本。在第二阶段计划期间，建设和测试 小样品室(如“毛细管”免疫测定仪)，用于 这些电化学传感器，可能导致快速， 自动化免疫分析需要非常少量的样本。

The overall objective of this project is to develop highly stable electrochemical probes for the monitoring of the reduced cofactor nicotinamide adenine dinucleotide (NADH), which is produced in many enzyme-based immunoassays. These sensors can measure NADH concentrations at low applied potentials, where electrochemical interferences and deactivating chemical reactions are avoided. The Phase I research will attempt to establish the feasibility of using these new sensors for the measurement of NADH produced in clinically important immunoassays. The development of an electrochemical probe which can selectively measure NADH concentrations could permit analysis of very small samples, which is of importance, for example, to clinical assays of neonatal blood samples. During the Phase II program, the construction and testing of small sample chambers (e.g., "capillary" immunoassays), for use with these electrochemical sensors, could lead to the development of rapid, automated immunoassays requiring very small amounts of sample.