AMPEROMETRIC GLUCOSE SENSOR WITH REDOX POLYMER MEDIATORS

该提案描述了新的酶为基础的设计和测试 用于葡萄糖的电流型传感器， 得到葡萄糖氧化酶的黄素氧化还原中心和电极， 通过供体-受体中继网络， 柔性聚合物 传感器应该有广泛的技术 在临床实验室、兽医健康等领域的应用 护理，研究实验室和食品工业。 因为 电极，研制可重复使用的体外血糖监测仪 (for糖尿病患者的自我监测）似乎在近期可行 未来 聚合物中继系统也适用于针型聚合物中继系统。 可用于皮下葡萄糖监测的探针。 的 第二阶段的拟议研究计划涉及高度发展 稳定、灵敏、选择性的葡萄糖传感器 新的聚合物电子传递系统，新的酶固定化方案， 以及新的电极配置和材料。

This proposal describes the design and testing of new enzyme-based amperometric sensors for glucose where the electrical communication between the flavin redox centers of glucose oxidase and an electrode is achieved via a network of donor-acceptor relays chemically bound to a highly flexible polymer. The sensors should have widespread technological applications in areas such as the clinical laboratory, veterinary health care, the research laboratory, and the food industry. Because the electrode, the development of a reusable in vitro blood glucose monitor (for self-monitoring by diabetic patients) appears feasible in the near future. The polymeric relay systems are also amenable to needle-type probes which could be useful for subcutaneous glucose monitoring. The proposed research plan for Phase II concerns the development of highly stable, sensitive and selective glucose sensors through a systematic study of new polymeric electron relay systems, new enzyme immobilization schemes, and new electrode configurations and materials.