ADVANCED BIOSENSORS FOR THE MEASUREMENT OF CHOLESTEROL

该方案描述了一种新型传感器的设计和测试 用于基于脱酯的总胆固醇的测量 胆固醇酯酶，由胆固醇氧化酶氧化，并介导 还原胆固醇氧化酶向电极的电子转移 新的氧化还原系统。因为这些氧化还原系统有非常负面的影响 形式电位，传感器可以在如下的电位下工作 常见的干扰物，如抗坏血酸和尿酸，不是 检测到。第一阶段的拟议研究计划涉及 通过以下方法开发胆固醇传感器： (1)多种新型单体和聚合物氧化还原的合成 可与胆固醇一起起到调节作用的系统 (2)基于胆固醇的胆固醇传感器的构建 酯酶、胆固醇氧化酶和这些电子转移介体； (3)胆固醇酯酶与胆固醇的共价结合 将氧化物酶转化为含介体的聚合物，以便进一步 增加氧化酶和介体之间的相互作用，并 提高了传感器的长期稳定性；(4)优化了传感器 传感器在以下方面：对胆固醇的快速反应， 对常见的电活性干扰不敏感，对 氧气、对生物液体中污垢的抵抗力和长传感器 一辈子。在第二阶段，这个小型传感器将与 生产便携式、自给式设备所需的电子设备。

This proposal describes the design and testing of a new type of sensor for the measurement of total cholesterol based on deesterification by cholesterol esterase, oxidation by cholesterol oxidase, and mediated electron transfer from reduced cholesterol oxidase to an electrode by novel redox systems. Because these redox systems have quite negative formal potentials, the sensors can be operated at a potential where common interferents, such as ascorbic acid and uric acid, are not detected. The proposed research plan for Phase I concerns the development of the cholesterol sensor through the following approach: (1) synthesis of a variety of new monomeric and polymeric redox systems which can serve as mediators in conjunction with cholesterol oxidase; (2) construction of cholesterol sensors based on cholesterol esterase, cholesterol oxidase, and these electron transfer mediators; (3) covalent attachment of cholesterol esterase and cholesterol oxidase to the mediator-containing polymers in order to further increase the interaction between the oxidase and the mediators, and to improve the long-term stability of the sensor; (4) optimization of the sensor with respect to the following: rapid response to cholesterol, insensitivity to common electroactive interferents, insensitivity to oxygen, resistance to fouling in biological fluids, and long sensor lifetime. During Phase II, this small sensor will be coupled with the necessary electronics to produce a portable, self-contained device.