COLD-ADAPATION OF HUMAN ROTAVIRUSES

部分人轮状病毒冷适应突变体的筛选 去年报告了重大的流行病学重要性。这些变种人 包括毒株D(VP4：1A；VP7：1)、DS-1(VP4：1B；VP7：2)、Wa×DS-1 (VP4：1A；VP7：2)和Wa x P(VP4：1A；VP7：3)。精选突变疫苗 目前正在进行临床评估。在试图提供 四种具有重要流行病学意义的VP7病毒的抗原性报道 血清型(G1、G2、G3和G4)，我们传代了一株Wa x ST3(VP4：1A；VP7：4) 在原代非洲绿猴肾细胞中的重组序列 亚最佳温度逐渐降低(30摄氏度，28摄氏度， 和26摄氏度)。每个突变体的三重空斑纯化 在该温度下进行第十代连续传代后 短文系列。

The selection of cold-adapted mutants of selected human rotaviruses of major epidemiologic importance was reported last year. These mutants included strain D (VP4:1A; VP7:1), DS-1 (VP4:1B; VP7:2), Wa x DS-1 (VP4:1A; VP7:2), and Wa x P (VP4:1A; VP7:3). Selected mutant vaccines are currently under clinical evaluation. In an attempt to provide antigenic coverage for each of the four epidemiologically important VP7 serotypes (G1, G2, G3, and G4), we passaged a Wa x ST3 (VP4:1A ; VP7:4) reassortant serially in primary African green monkey kidney cells at progressively lower suboptimal temperature (30 degrees C, 28 degrees C, and 26 degrees C). Triple plaque purification of each mutant selected after tenth serial passages is in progress at the temperature of that passage series.