USE OF ORBITAL ROTATION TO INCREASE SENSITIVITY OF SHELL VIAL VIRAL CULTURES

壳瓶培养试验大大缩短了 许多病毒的实验室检测。有报道称， 单独使用带壳小瓶，不同时使用常规试管 培养物可能导致实验室遗漏一小部分 病毒滴度较低的阳性标本。的一种方法 增加外壳小瓶的灵敏度将有利于 临床实验室的费用和时间花费在使用 传统的试管培养将被保存。已经显示 滚动和轨道运动对疱疹病毒有积极作用 在试管培养物和带壳小瓶中复制。我们希望展示 通过在轨道旋转器上孵育壳小瓶病毒培养物， 只有阳性培养物的数量增加， 与静止培养相比， 积极性和积极性程度的增加将被看到。

The shell vial culture assay has greatly decreased the time to laboratory detection of many viruses. Reports have suggested that the use of shell vials alone without concomitant conventional tube cultures may result in the laboratory missing a small percentage of positive specimens which have a low titer of virus. A method which increases the sensitivity of the shell vial would be advantageous to the clinical laboratory in that the expense and time expended in using conventional tube cultures would be saved. It has been shown that rolling and orbital motion have a positive effect on herpesvirus replication in tube cultures and shell vials. We hope to demonstrate that by incubating shell vial viral cultures on an orbital rotator not only an increase in the number of positive cultures will result as compared to stationary cultures, but also a decrease in the time to positivity and an increase in degree of positivity will be seen.