STUDIES OF T LYMPHOCYTE FUNCTION IN TRANSPLANTATION

人外周血辅助性T细胞(Th)体外反应的研究 白细胞(PBL)对人类白细胞抗原同种异体抗原的调节 TH抗原提呈细胞(APC)途径：1)CD4+Th和自体APC (2)CD4+Th和同种异体APC；以及3)CD8+Th和 同种异体APC(CD8-AAPC)这些问题有不同程度的敏感性 环孢素A(CsA)和环孢素A(CsA)免疫抑制作用的途径 FK506，因此，CD4-SapC是最敏感的，而CD4-AAPC是 最不敏感的是，当这些药物从 健康的捐赠者。 我们检测了150多个人肾的体外Th功能。 同种异体移植受者接受多种药物免疫抑制治疗。我们的 结果表明，只有功能完整的CD4-SapC的存在 与慢性或急性移植物排斥反应相关的通路。我们的发现 提示这种方法可以用来监测移植物的状态 器官移植接受者。

The in vitro T helper cell (Th) response of human peripheral blood leukocytes (PBL) to HLA alloantigens is mediated by three distinct Th-antigen presenting cell (APC) pathways: 1) CD4+ Th and autologous APC (CD4-sAPC); 2 ) CD4+ Th and allogeneic APC (CD4-aAPC); and 3) CD8+ Th and allogeneic APC (CD8-aAPC). There is a hierarchy of sensitivity of these pathways to the immunosuppressive effects of cyclosporin A (CsA) and FK506, such that the CD4-sAPC is the most sensitive and CD4-aAPC is the least sensitive, when these drugs are added to cultures of PBL from healthy donors. We tested the in vitro Th function of more than 150 human renal allograft recipients on multi-drug immunosuppressive therapy. Our results indicate that only the presence of a functionally intact CD4-sAPC pathway correlated with chronic or acute graft rejection. Our findings suggest that this approach can be used to monitor the graft status of organ transplant recipients.