HEPATOCELLULAR CARCINOMA IN CHIMPANZEES

肝细胞癌，慢性乙型或丙型肝炎感染的偶然后果 人类中的病毒(乙肝病毒、丙型肝炎病毒)只有三种被描述过 黑猩猩。肝细胞癌和邻近非肿瘤性肝脏的尸检 其中用抗生物素-生物素-复合体免疫组织化学方法进行了研究。 方法。两种肿瘤均为分化较好的小梁型肝癌。都不是 黑猩猩有活动性的乙肝病毒感染；一只患有慢性非A，非B 肝炎(很可能是由丙型肝炎病毒引起的)。 转化生长因子-α在两种黑猩猩70%的肝细胞癌细胞(大多数 其他肝癌细胞均为阴性)。两只黑猩猩的Hcc染色 P53阴性；这可能表明没有突变型P53 蛋白。然而，一只黑猩猩有几个显微镜下的病灶 含有少量细胞核的非肿瘤性肝脏中的肝细胞(1%) P53阳性，可能是由于 肿瘤外的突变事件。椭圆形细胞，以椭圆形为特征 胞核、清晰稀少的细胞质以及体积小于 在两只黑猩猩的肝组织中都观察到了成熟的肝细胞 转化生长因子-α呈强阳性表达。

HCC, an occasional outcome of chronic infection with hepatitis B or C viruses (HBV, HCV) in humans, has been described in only three chimpanzees. HCC and adjacent nontumorous liver from autopsies from two of these were studied using an avidin-biotin-complex immunohistochemical method. Both tumors were well-differentiated trabecular HCCs. Neither chimpanzee had active HBV infection; one had chronic non-A, non-B hepatitis (most likely due to HCV). TGF-alpha stained strongly in >70% of HCC cells of both chimpanzees (most other HCC cells were negative). The HCCs of both chimpanzees stained negatively for p53; this probably indicates the absence of mutant p53 protein. However, one chimpanzee had several microscopic foci of hepatocytes in the nontumorous liver that contained a few nuclei (<1%) that stained positively for p53, possibly the result of additional mutational events outside the tumor. Oval cells, characterized by oval nuclei, clear and scanty cytoplasm, and dimensions that are smaller than mature hepatocytes, were observed in liver tissues from both chimpanzees in the present study and were stained strongly positive for TGF-alpha.