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DEVELOPMENT OF SYSTEMS TO DETECT PCR CONTAMINATION

DEVELOPMENT OF SYSTEMS TO DETECT PCR CONTAMINATION
PCR 污染检测系统的开发
批准号：
3853587
负责人：
E TABOR
金额：
$0.0万
依托单位：
DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3853587
关键词：
biomedical equipment safety diagnosis quality /standard hepatitis B virus group human tissue liver disorder diagnosis polymerase chain reaction virus classification

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相关文献

中文摘要
翻译
聚合酶链反应（PCR）可用于检测小 B肝炎病毒（HBV）DNA的数量，通常通过测试一个 或两个基因序列，在肝脏和血清中， HBV的可检测标志物。 有时需要区分 从由于污染引起的假阳性结果中检测HBV DNA 先前扩增的PCR产物的残留。 偶尔使用 使用针对三种HBV基因的部分的引物组的“多靶”PCR， C、S和X，可以同时验证没有污染， 提高PCR的准确性。
英文摘要
The polymerase chain reaction (PCR) can be used to detect small quantities of hepatitis B virus (HBV) DNA, generally by testing for one or two gene sequences, in liver and serum from patients without detectable markers of HBV. It is sometimes necessary to distinguish the detection of HBV DNA from false-positive results due to contamination by carry-over of previously amplified PCR products. Occasional use of "multi-target" PCR using primer sets for portions of the three HBV genes, C, S, and X, simultaneously can verify the lack of contamination by carry-over and enhance the accuracy of PCR.
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会议论文
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