BIOPHYSICAL INSTRUMENTATION AND METHODOLOGY

该项目旨在开发新的仪器和方法，并 改进现有的仪器和技术，以进行表征 生物大分子及其相互作用的研究。 分析超速离心法及其辅助技术和方法 使用适用于以下各项的数学建模进行数据分析 技术是人们感兴趣的主要领域。 数学建模在数学问题中的应用研究 超速离心分析和新的检测方法的发展 实验和数据分析仍在继续。特别是：(1) 泥沙浓度依赖性的广泛研究 在非常高的蛋白质浓度下的系数一直在继续。(2) 隐式约束法在结构分析中的应用 同时实现了同质和异质关联。 (3)提出了一种求取上、下限的方法。 糖蛋白组成时糖蛋白的相对分子质量 碳水化合物部分未知。(4)发展了一种新的方法。 测量蛋白质与具有异质性的受体的相互作用 分子量分布。(5)一种新的分析方法 开展了泥沙沉降速度实验。

The project is designed to develop new instrumentation and methods, and to improve existing instrumentation and techniques, for the characterization of biological macromolecules and the study of their interactions. Analytical ultracentrifugation, the techniques ancillary to it, and methods of data analysis using mathematical modeling appropriate for these techniques are the major areas of interest. Studies on the application of mathematical modeling to problems of ultracentrifugal analysis, and the development of new methods of performing experiments and analyzing data, have continued. In particular: (1) Extensive studies on the concentration dependence of sedimentation coefficients at very high protein concentrations have been continued. (2) Application of the method of implicit constraints to the analysis of simultaneous homogeneous and heterogeneous associations has been achieved. (3) A method has been developed for obtaining the upper and lower limits of the molecular weight of a glycoprotein when the composition of the carbohydrate portion is unknown. (4) A method has been developed for measuring the interaction of proteins with receptors having a heterogeneous molecular weight distribution. (5) A new method for the analysis of sedimentation velocity experiments has been developed.