MYELOTOXICITY INDUCED IN B6C3F1 MICE BY METHYL ISOCYANATE INHALATION EXPOSURE

吸入4天(每天6小时)对0、1和 3ppm甲基异氰酸酯对女性骨髓参数的影响 分别于染毒后5、8、21天和1年对小鼠进行检测。 如证据所示，MIC暴露与骨髓有关 通过细胞减少，抑制多能干细胞(cfu-S)， 粒-巨噬系祖细胞(CFU-C)与红系祖细胞 (CFU-E)。造血细胞参数恢复到 1ppm剂量组21天恢复正常，但3ppm剂量组不正常 剂量组。MIC是一种高活性的化学物质，似乎 直接作用于鼻腔衬里上皮 空洞和主要呼吸道，没有组织学证据 具有系统性的影响。对骨骼没有明显的影响 急性髓系白血病小鼠1年后骨髓细胞数和CFU-C的变化 暴露在3ppm和10ppm的剂量下，持续2小时。总之， MIC暴露可导致衬里上皮细胞急性死亡 有一过性改变的鼻道和主要呼吸道 骨髓参数可能与肺组织 直接损伤或通过胸腺继发损伤。这些 结果发表在环境杂志上。健康观察。72，第143-148页， 1987年。

The effects of a 4-day inhalation exposure (6 hr/day) to 0, 1 and 3 ppm methyl isocyanate (MIC) on bone marrow parameters in female mice were examined at 5, 8, 21 days and 1 year following exposure. The MIC exposure was associated with the bone marrow as evidenced by hypocellularity, suppression of pluripotent stem cells (CFU-S), granulocyte-macrophage progenitors (CFU-C) and erythroid precursors (CFU-E) in both dose groups. Hematopoietic parameters returned to normal by 21 days in the 1 ppm dose group, but not in the 3 ppm dose group. MIC is a highly reactive chemical that appears to exert its effect directly on the lining epithelium of the nasal cavity and major airways, and there was no histological evidence of a systemic effect. There was no significant effect on bone marrow cellularity and CFU-C in mice 1 year following acute exposure at the doses of 3 and 10 ppm for 2 hours. In conclusion, MIC exposure appears to cause acute cell death of lining epithelium of the nasal passages and major airways with transient alterations of bone marrow parameters that are likely related to the pulmonary injury either directly or secondary through the thymus. These results were publicated in Environ. Health Persp. 72, p. 143-148, 1987.