PHYSICAL CHEMISTRY OF BIOLOGICAL MACROMOLECULES

这个项目的目的是研究一种广泛的 各种生物大分子，目的是将它们相互关联 性质与大分子的结构和功能有关。这个 重点放在这些物质相互作用的热力学上。 大分子以及它们的分子大小和形状。分析性 主要研究内容为超速离心法和数学模型法 使用的技术。 纤维蛋白原与其他相关蛋白相互作用的研究 血液凝结和纤溶一直在继续，目前正在处理 纤维蛋白原与血浆、血小板XIII因子及 纤溶酶原与纤维蛋白原D、E片段的相关性。 对蓖麻毒素的研究包括对抗蓖麻毒素的单抗的研究。 蓖麻毒素B链、该抗体与蓖麻毒素的相互作用及其作用 蓖麻毒素的体外和体内毒性研究。对二十七位S的研究 胞苷的中间体参与了对其作用机制的研究。 这种形式的网状蛋白的形成及其与形成的关系 笼形篮子。 对突触素的研究主要是为了确定分子 该蛋白质单体形式的重量，并确定是否 它所经历的聚集是可逆的、不可逆的或组合的 两者都是。细胞粘附肽的研究正朝着以下方向发展 确定它们的自缔合模式，测量热力学 表征其自关联的参数，测量 表征其自缔合的热力学参数，检验 他们之间可能的互动，并试图建立一个 它们的联想行为与它们的一级结构之间的关系。

The purpose of this project is to study the physical properties of a wide variety of biological macromolecules with the goal of correlating these properties to the structure and function of the macromolecules. The emphasis is on the thermodynamics of the interactions of these macromolecules and on their molecular size and shape. Analytical ultracentrifugation and mathematical modeling are the principal research techniques used. Studies on the association of fibrinogen with other proteins involved in blood clotting and fibrinolysis have been continued and currently deal with the association of fibrinogen with plasma and platelet Factor XIII and with the association of plasminogen with the D and E fragments of fibrinogen. Research on ricin has involved studies on a monoclonal antibody to the ricin B chain, the interaction of this antibody with ricin, and its effect on ricin toxicity both in vitro and in vivo. Research on the 27 S intermediate form of clathrin involved studies on the mechanism of formation of this form of clathrin and its relationship to the formation of clathrin baskets. Studies on synapsin have been directed toward determining the molecular weight of the monomeric form of this protein and determining whether the aggregation which it undergoes is reversible, irreversible or a combination of both. Studies on cell adhesion peptides have been directed toward determining their mode of self-association, measuring the thermodynamic parameters characterizing their self-association, measuring the thermodynamic parameters characterizing their self-association, examining their possible interactions with each other and attempting to establish a relationship between their associative behavior and their primary structure.