USE OF ORBITAL ROTATION TO INCREASE SENSITIVITY OF SHELL VIAL VIRAL CULTURES

贝壳小瓶培养试验大大缩短了化验时间 检测到许多病毒。有报道称，壳牌的使用 单独使用小瓶而不同时进行常规试管培养可能导致 实验室遗漏了一小部分阳性样本，这些样本 低滴度的病毒。一种方法，它增加了对 壳牌小瓶对临床实验室有利，因为 使用常规试管培养所花费的费用和时间将是 得救了。已经证明，滚转和轨道运动具有正性。 在试管培养和贝壳培养瓶中对疱疹病毒复制的影响。我们 希望通过将贝壳小瓶病毒培养在 眼眶旋转器，不仅阳性培养数量增加 与静止培养相比，不仅会减少 积极的时间和积极程度的增加将是 看到了。然而，对该方法的进一步分析表明， 由于资源和空间的限制，这种方法不会 在我们的环境下是可行的。因此，该项目已被终止。

The shell vial culture assay has greatly decreased the time to laboratory detection of many viruses. Reports have suggested that the use of shell vials alone without concomitant conventional tube cultures may result in the laboratory missing a small percentage of positive specimens which have a low titer of virus. A method which increases the sensitivity of the shell vial would be advantageous to the clinical laboratory in that the expense and time expended in using conventional tube cultures would be saved. It has been shown that rolling and orbital motion have a positive effect on herpesvirus replication in tube cultures and shell vials. We hoped to demonstrate that by incubating shell vial viral cultures on an orbital rotator, not only an increase in the number of positive cultures would result as compared to stationary cultures, but also a decrease in the time to positivity and an increase in degree of positivity would be seen. Further analysis of the methodology, however, indicated that, because of resource and space limitations, the methodology would not be practicable in our setting. The project has therefore been terminated.