TIME RESOLVED MEASUREMENTS OF TRYPTOPHAN MUTANTS OF MYOGLOBIN

有机分子电子跃迁的理论计算 不同激发模式的分子允许人们预测 截面和多光子激发的基本各向异性。 对于双光子，NADH的基本各向异性接近0.54 从690-740 nm激发，这比0.37的值高46%， 观察到单光子激发。 这是一个更高的观察 双光子激发的各向异性与INDO/SDCI一致 单光子和双光子跃迁的计算。 NADH显示a 双光子激发的低截面，这表明， 对于双光子荧光，来自NADH的荧光将是可忽略的 显微镜检查，并不会显着干扰观察 用于标记细胞的其他外源性探针的TPIF。

Theoretical calculations of electronic transitions in organic molecules for different modes of excitation allow one to predict the cross-section and fundamental anisotropy for multi-photon excitation. The fundamental anisotropy of NADH is near 0.54 for two-photon excitation from 690-740 nm, which is 46% higher than the value of 0.37 observed for one-photon excitation. This observation of a higher anisotropy with two-photon excitation was consistent with INDO/SDCI calculation of the one- and two-photon transition. NADH displays a low cross-section for two photon excitation, which suggest that fluorescence from NADH will be negligible for two-photon fluorescence microscopy and will not significantly interfere with observations of TPIF of other extrinsic probes used to label cells.