DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

美国国立卫生研究院动物遗传资源（NIHAGR）保存了几种动物物种， 以及一个物种中许多无价的基因型， 在生物医学研究中。 为了保护这种种质， 作为一种经济手段建立，以无限期地保存这些股票 以及啮齿动物和兔子的品系。 胚胎冷冻保存有其他 基因型管理的好处，如防止细菌损失 疾病、生殖失败、自然灾害或遗传 污染. 冷冻库最大限度地减少由于遗传漂变的变化， 提供一种资源，从该资源周期性地再衍生出定义的基因型 重新标准化基础繁殖群体。 作为公认的 实验室动物物种的储存库，NIHAGR定期提供 在整个生物医学研究界应要求培育细胞核。 冷冻保存的胚胎代表了一种 更简单、更人性化、更容易运输所需种质的方法 国际吧 胚胎处理的另一个重要影响，冷冻保存， 和转移程序是为了消除一些垂直传输 鼠疾病 基因型和物种差异继续影响 这个效率就是努力。

The NIH Animal Genetic Resource (NIHAGR) maintains several animal species and many invaluable genotypes within a species, which are used as models in biomedical research. To protect this germ plasm, an embryo bank was established as an economical means to indefinitely preserve these stocks and strains of rodents and rabbits. Cryopreservation of embryos has other benefits for genotype management such as safeguarding against loss of germ plasm from disease, reproductive failure, natural catastrophe or genetic contamination. The cryobank minimizes changes due to genetic drift by providing a resource from which to periodically rederive defined genotypes for restandardization of foundation breeding colonies. As a recognized repository for laboratory animal species, the NIHAGR routinely provides breeding nuclei upon request throughout the biomedical research community. Rather than shipping live animals, cryopreserved embryos represent a simpler, more humane and easier method to transport requested germ plasm worldwide. Another important impact of embryo handling, cryopreservation, and transfer procedures is to eliminate the vertical transmission of some murine diseases. Genotype and species differences continue to impact on the efficiency of this is effort.