CHARACTERIZATION OF BMV REPLICATION COMPLEX

雀麦花叶病毒(BMV)是类似甲病毒的一种 是一种感染植物的(+)链RNA病毒。烟草花叶病毒RNA 复制完全发生在感染细胞的细胞质中，并且 需要2个病毒编码的蛋白质和几个宿主因子。研究 表明RNA复制发生在BMV和相关病毒的 与细胞质膜的关系及其特化 病毒诱导的细胞质小泡可能参与其中。我们正在使用 进一步确定亚细胞特征的电子显微镜 这些特化小泡的定位和组成。冷冻固定 这些技术将在这些研究中被证明是有用的，以帮助 膜超微结构的保存。我们希望将其描述为 病毒诱导的大麦原生质体中的小泡，大麦的自然宿主 BMV，以及在酿酒酵母中，一种遗传上易驯化的 BMV复制的系统。

Brome mosaic virus (BMV) is a member of the alphavirus-like superfamily and is a plant-infecting, (+)-strand RNA virus. BMV RNA replication occurs entirely in the cytoplasm of infected cells and requires 2 virally-encoded proteins and several host factors. Studies of BMV and related viruses indicate that RNA replication occurs in association with cytoplasmic membranes and that specialized virus-induced cytoplasmic vesicles are likely involved. We are using electron microscopy to characterize further the subcellular localization and makeup of these specialized vesicles. Cryofixation techniques will prove useful in these studies to help in the preservation of membrane ultrastructure. We hope to characterize virus-induced vesicles in both barley protoplasts, the natural host of BMV, and also in Saccharomyces cerevisia, a genetically tractable system in which BMV replicates.