SYNCHROTRON CRYSTALLOGRAPHY BEAMLINE X9A STRUCTURE FUNCTION STUDIES

将从该设施中受益的特定结构项目， 由于弱衍射晶体或难以获得多个 衍生物，包括调节真核RNA的辅助蛋白 聚合酶，真核DNA聚合酶系统的组成部分，E. Coli RNA聚合酶，离子通道的调节和结构， 将肌动蛋白细丝、SH2和SH3交联、捆绑和切断的蛋白质 模块、磷酸酶、细胞周期蛋白依赖性蛋白激酶和酰基载体 蛋白质依赖的酰基转移酶。这些结构性结果将 对细胞生物学中最重要的领域有重大影响， 包括真核和原核转录调控、信号 转导，真核DNA复制，原核细胞壁 肌动蛋白细胞骨架的生物合成、调控、机制 膜蛋白功能与细胞周期调控。这些结构 结果也将直接使我们的即时计划受益 合作者，最重要的是将提供结构性的 对今后的工作至关重要的基础，由大量的 从事这些分支学科研究的生物学家。

Specific structural projects that will benefit from this facility, due to weakly diffracting crystals or difficulty in obtaining multiple derivatives, include the accessory proteins regulating eukaryotic RNA polymerase, components of the eukaryotic DNA polymerase system, E. coli RNA polymerase, regulation and structure of ion channels, proteins that crosslink, bundle and sever actin filaments, SH2 and SH3 modules, phosphatases, cyclin-dependent kinases and acyl carrier protein-dependent acyl transferases. These structural results will have major impact on areas of central importance in cell biology, including eukaryotic and prokaryotic transcriptional control, signal transduction, eukaryotic DNA replication, prokaryotic cell wall biosynthesis, regulation of the actin cytoskeleton, mechanisms of membrane protein function and cell cycle control. These structural results will also directly benefit the programs of our immediate collaborators, and, most importantly will provide the structural foundation that is essential for future work by the large number of biologists working in each of these sub-disciplines.