CORE-SCIENTIFIC

科学核心的存在是为了促进内部的科学项目 这个项目。该组织提供的服务、材料和试剂 科学核心是纯化的羊瘙痒病毒，PrP抗体，人工合成的 肽和寡核苷酸，重组PrP变异体，转基因小鼠 筛查以及化学和生化分析服务。这个核心 将由迈克尔·鲍德温博士执导，他是 拥有丰富的经验，曾指导过一家 他在伦敦大学工作了14年，致力于 在过去的六年里，我们深入研究了普里恩的化学和生物化学 蛋白。纯化的普鲁恩的制备已经在这里进行了。 实验室已经有十多年了。近年来，这一计划一直在进行 由Hana Serban女士监督。她将监督PrP的生产 抗体，对项目1-4和核心C 2的成功至关重要 SRA都有相当丰富的经验，将合成多肽和 寡核苷酸。项目3-5需要多肽，项目3-5需要 抗体的产生。项目3和项目3将需要寡核苷酸 4和这个核心用于转基因小鼠的生产和筛选 并为分子生物学提供了各种其他工具。 重组PrP变异体将由Michael博士在培养中表达 斯科特，一位杰出的分子生物学家，用于项目4和5。 对项目3至关重要的转基因小鼠的筛选将 由Ruth Koehler女士携带。一个SRA将进行化学和 生化分析将在抗体研究中发挥重要作用 生产和转基因小鼠的生产，也将使项目受益 2比5。所有这些活动目前都在进行中 在这个实验室里成功地有了经验丰富的 已经上岗的人员。

The Scientific Core exists to facilitate the scientific projects within the program. The services, materials and reagents provided by the Scientific Core are purified scrapie prions, PrP antibodies, synthetic peptides and oligonucleotides, recombinant PrP variants, transgenic mouse screening and a chemical and biochemical analytical service. This core will be directed by Dr. Michael Baldwin, an analytical chemist of considerable experience who directed a core service laboratory in the University of London for 14 years and who has devoted himself for the last six years to reach into the chemistry and biochemistry of the prion protein. The preparation of purified prions has been carried out in this laboratory for more than a decade. In recent years this program has been supervised by Ms. Hana Serban. She will supervise the production of PrP antibodies, crucial to the success of projects 1-4 and Core C. Two SRA's, both having considerable experience, will synthesize peptides and oligonucleotides. Peptides are required for projects 3-5, and for antibody production. oligonucleotides will be needed for projects 3 and 4 and this core for transgenic mouse production and for screening these mice as well as providing a variety of other tools for molecular biology. Recombinant PrP variants will be expressed in culture by Dr. Michael Scott, an outstanding molecular biologist, for use in projects 4 and 5. The screening of transgenic mice which is essential for project 3 will be carried by Ms. Ruth Koehler. An SRA will carry out chemical and biochemical analysis, which will play an important role in antibody production and transgenic mouse production and will also benefit projects 2-5. All of these activities are currently being carried out very successfully in this laboratory and there are highly experienced personnel already in post.