ACQUISITION OF A MALDI/TOF MASS SPECTROMETER SYSTEM

该提案要求为购买PE Biosystems Voyager-DE STR MALDI/TOF质谱仪和数据系统提供资金。这种高分辨率仪器配备了反射分析仪，能够通过源后衰变对分子离子和碎片离子形式进行精确的质量分析。它将位于纽约州立大学石溪分校质谱仪设施内，并将显著促进来自纽约州立大学石溪分校和布鲁克海文国家实验室的14位杰出科学家的研究目标和实验室生产力，他们构成了主要用户群。质谱仪系统将为这些研究人员提供重要的分析生物分子的新技术，这些技术目前在当地是无法获得的，而且很难从其他组织获得。MALDI/TOF在具有重要生物医学意义的分子结构分析方面是无与伦比的，包括生物聚合物，如蛋白质、寡核苷酸、寡糖和各种非挥发性、不稳定或热不稳定的化合物。蛋白质分子离子的精确质量分析和蛋白质水解片段的测定将被用于通过自动计算机搜索基于互联网的数据库来鉴定蛋白质。对一些研究者来说，低聚脱氧核苷酸、修饰低聚物和dna -蛋白质复合物的负离子检测将是必不可少的。大型多孔板的自动化样品分析将确保仪器的高通量和最佳利用率。仪器将由USB质谱仪设施的训练有素的人员操作和维护，主任将安排日常操作并为用户提供技术专业知识。一个咨询委员会将确保与主要用户和其他小灵通支助的调查人员取得联系，并将负责该仪器的长期运行。石溪管理部门为购买仪器、安装和运行预期寿命做出了重大贡献。

This proposal requests funds for the purchase of a PE Biosystems Voyager-DE STR MALDI/TOF Mass Spectrometer and Data System. This high resolution instrument is equipped with a reflectron analyzer and is capable of accurate mass analysis of molecular ions and fragment ions forms through post source decay. It will be located in the SUNY- Stony Brook Mass Spectrometer Facility and will significantly promote the research objectives and laboratory productivity of fourteen distinguished scientists from SUNY-Stony Brook and Brookhaven National Laboratory who comprise the major users group. The mass spectrometer system will make directly available to these investigators important new techniques for the analysis of biomolecules which are presently unavailable locally and inaccessible or difficult to obtain from other organizations. MALDI/TOF is unsurpassed for the structural analysis of molecules with critical biomedical consequences, including biopolymers such as proteins, oligonucleotides, and oligosaccharides and a diverse group of compounds that are non-volatile, labile, or thermally unstable. Accurate mass analysis of protein molecular ions and determination of proteolytic fragments from proteins will be employed to identify proteins by automated computer searching internet-based databanks. Negative ion detection for oligodeoxynucleotides, modified oligomers, and DNA-protein complexes will be essential for several investigators. Automated sample analysis from large multi-well plates will ensure high throughput and optimal utilization of the instrument. The instrument will be operated and maintained by trained personnel at the USB Mass Spectrometer Facility, and the Director will schedule daily operation and provide technical expertise for the users. An advisory committee will ensure access to the major users and other PHS- supported investigators and will be responsible for the long term operation of the instrument. The Stony Brook administration has made a significant contribution toward the purchase of the instrument and to the installation and operation over its expected lifetime.