CORE--SCIENTIFIC

这个核心部分的主要目标是建立一个化学分析和寡核苷酸合成的中央设施，为项目研究项目提供必要的服务。核心项目的科学功能由伊登博士指导，包括自动合成和纯化修饰的寡聚脱氧核苷酸，以及复杂的高效液相和质谱仪分析。在一个核心设施中既包括分析方面，又包括合成方面，确保所有服务都可免费用于项目研究，并妥善维护尖端仪器。寡聚脱氧核苷酸的合成和纯化对该项目的所有研究人员来说都是至关重要的。这些服务可以作为一个利用训练有素的人员的集中化设施来最有效地完成。质谱学将在DNA加合物和含有新加合物的合成寡核苷酸的分析中发挥关键作用。两个重要的新仪器，一个是微质量四质谱LC/MS/MS系统和一个微质量平台LC/MS仪器，最近加入了质谱学设施，将是这些分析的基础。毛细管区带电泳法的发展将是鉴定合成低聚物中杂质的关键，并将在使用中补充高效液相色谱技术。

The primary objective of this Core component is to establish a central facility for chemical analysis and oligodeoxynucleotide synthesis that will provide essential services for Program research projects. The scientific functions of the Core Project are directed by Dr. Iden and include automated synthesis and purification of modified oligodeoxynucleotides and sophisticated HPLC and mass spectrometer analysis. Including both the analytical and synthetic aspects in a core facility ensures that all services will be freely available for project research and sophisticated instruments will be maintained properly. Synthesis and purification of oligodeoxynucleotides is critical for all investigators in the Program. These services can most effectively be accomplished as a centralized facility utilizing trained personnel. Mass spectrometry will play a critical role in the analysis of DNA adducts and synthetic oligodeoxynucleotides containing new adducts. Two important new instruments, a Micromass Quattro LC/MS/MS system and a Micromass Platform LC/MS instrument, recently added to the Mass Spectrometry Facility, will be fundamental for these analyses. Development of capillary zone electrophoresis will be essential for identification of impurities in synthetic oligomers and will complement HPLC techniques in use.