HIGH RES DATA COLLECTION ON NATIVE & COMPLEXED SULFITE REDUCTASE HEMOPROTEIN

亚硫酸盐还原酶血红蛋白(SiRHP)构成催化 多组分复合酶亚硫酸盐还原酶的亚基。 亚硫酸盐还原酶负责六个电子的还原 硫磺生物同化中的亚硫酸盐转化为硫化物。什么时候 由人工给电子体提供的KDa单体SiRHP是 完全能够进行由该络合物执行的还原化学。 血球蛋白亚基利用一组独特的辅因子来 将电子转移到衬底。氧化酶的结构 目前正在建设和完善中。原子分辨率信息 这种酶的各种络合和还原形式应该有助于 对生物还原过程中所涉及的化学问题有深刻的认识 低分子化合物。

Sulfite Reductase Hemoprotein (SiRHP) constitutes the catalytic subunit of the multicomponet enzyme complex Sulfite Reductase. Sulfite Reductase is responsible for the six electron reduction of sulfite to sulfide in the biological assimilation of sulfur. When supplied with artificial electron donors, SiRHP, a 64 KDa monomer, is fully capable of the reductive chemistry performed by the complex. The hemoprotein subunit utilizes a unique assembly of cofactors to transfer electrons to substrate. The structure of the oxidized enzyme is currently being built and refined. Atomic resolution information on this enzyme in its various complexed and reduced forms should lend great insight into the chemistry involved in the biological reduction of low molecular weight compounds.